Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, 88 East Wenhua Road, Ji'nan, Shandong, China.
Endocrine. 2011 Oct;40(2):187-95. doi: 10.1007/s12020-011-9477-y. Epub 2011 May 5.
Sloan-Kettering virus gene product (Ski) is an unique nuclear pro-oncoprotein and belongs to the ski/sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski/SnoN are important transcription regulators of the transforming growth factor-β (TGF-β) superfamily and function mainly through heterodimers. Since TGF-β superfamily are key regulators of follicle development and it has been previously shown that SnoN is also vital to follicle development, this research was conducted to clarify the relationship between Ski expression and mouse follicular development, in ovaries of neonatal and gonadotropin-induced immature mice by immunohistochemical and real-time PCR techniques. In postnatal mice, positive staining for Ski was highly detected in oocyte nuclei at postnatal day 1. With follicular development, the localization moved gradually from oocyte nuclei to perinuclear space and the total levels decreased. During the estrous cycle, Ski expression was apparent at proestrus and estrus, faint at metestrus, highest at diestrus. After injection of gonadotropin, Ski was found in perinuclear space and weak in oocyte nuclei. Following the initiation of luteinization, the expression of Ski was found in corpus luteum. Real-time PCR results also showed that Ski mRNA expression was opposite to ovulation-related genes during the cumulus expansion, with the development of the follicles, its expression level decreased. Ski is expressed in a specific manner during follicle development, ovulation and luteinization. So Ski might play essential roles in these processes especially during early follicular development.
斯隆-凯特琳病毒基因产物（Ski）是一种独特的核原癌蛋白，属于 Ski/sno 原癌基因家族。Ski 在多种细胞类型中发挥多种作用，当高水平表达时，它既能诱导致癌转化，又能诱导终末肌肉分化。Ski/SnoN 是转化生长因子-β（TGF-β）超家族的重要转录调节因子，主要通过异二聚体发挥作用。由于 TGF-β 超家族是卵泡发育的关键调节因子，并且之前已经表明 SnoN 对卵泡发育也至关重要，因此这项研究旨在通过免疫组织化学和实时 PCR 技术阐明 Ski 表达与小鼠卵泡发育的关系，在新生和促性腺激素诱导的未成熟小鼠的卵巢中。在新生小鼠中，Ski 在出生后第 1 天的卵母细胞核中高度检测到阳性染色。随着卵泡的发育，定位逐渐从卵母细胞核转移到核周空间，总水平下降。在动情周期中，Ski 在发情前期和发情期表达明显，在动情后期和间情期表达微弱。注射促性腺激素后，Ski 被发现在核周空间，卵母细胞核中的表达较弱。黄体化开始后，在黄体中发现 Ski 的表达。实时 PCR 结果还表明，在卵丘扩展过程中，Ski mRNA 表达与排卵相关基因相反，随着卵泡的发育，其表达水平降低。Ski 在卵泡发育、排卵和黄体化过程中以特定的方式表达。因此，Ski 可能在这些过程中发挥重要作用，尤其是在早期卵泡发育过程中。
