[采用聚丙烯酰胺凝胶电泳法对3种酶系统表型的测定]
[The determination of the phenotypes of 3 enzyme systems by the polyacrylamide gel electrophoresis method].
作者信息
Svirskiĭ M S, Kolmakova M A
出版信息
Sud Med Ekspert. 1990 Jan-Mar;33(1):37-8.
PMID:2139982
Abstract
Possibility of combined determination of phenotypes of three enzyme system (glyoxylase-1, phosphoglucomutase and 6-phosphogluconate dehydrogenase) in the apparatus with two vertical blocks of polyacrilamide gel (PAAG) was shown. The method is based on the principle of combination of differently composed blocks of PAAG and single universal electrode buffer. High productivity and economy of the method equally with good quality of resulting phoregrams make this method useful for medicolegal practical work.
摘要
已证实在配备有两个垂直聚丙烯酰胺凝胶（PAAG）块的仪器中联合测定三种酶系统（乙二醛酶-1、磷酸葡萄糖变位酶和6-磷酸葡萄糖酸脱氢酶）表型的可能性。该方法基于不同组成的PAAG块与单一通用电极缓冲液相结合的原理。该方法的高生产率和经济性以及所得电泳图谱的良好质量使其适用于法医学实际工作。
相似文献
1
[The determination of the phenotypes of 3 enzyme systems by the polyacrylamide gel electrophoresis method].[采用聚丙烯酰胺凝胶电泳法对3种酶系统表型的测定]
Sud Med Ekspert. 1990 Jan-Mar;33(1):37-8.
2
Glyoxalase I typing and phosphoglucomutase-1 subtyping of a single hair.单根毛发的乙二醛酶I分型和磷酸葡萄糖变位酶-1亚型分型
J Forensic Sci. 1987 Sep;32(5):1175-81.
3
Phosphoglucomutase1 (PGM1) and 6-phosphogluconate dehydrogenase (PGD) types in the human hairbulb.
Forensic Sci. 1978 Mar-Apr;11(2):135-8. doi: 10.1016/s0379-0738(78)80007-3.
4
Multiple enzyme typing of the sheath cells associated with the root of a single human head hair.对一根人类头发生根处相关鞘细胞的多种酶分型
J Forensic Sci Soc. 1982 Apr;22(2):203-9. doi: 10.1016/s0015-7368(82)71472-0.
5
An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I.一种用于磷酸葡萄糖变位酶-1、酯酶D或乙二醛酶I分型的琼脂糖凝胶电泳方法。
J Forensic Sci. 1985 Oct;30(4):1216-20.
6
Phenotyping of eight erythrocytic enzymes in one acrylamide gel.在一块丙烯酰胺凝胶中对八种红细胞酶进行表型分析。
Am J Clin Pathol. 1979 Jun;71(6):672-6. doi: 10.1093/ajcp/71.6.672.
7
[Demonstration of glyoxalase I (EC 4.4.1.5) in starch gel electrophoresis].[淀粉凝胶电泳中乙二醛酶I（EC 4.4.1.5）的显示]
Med Lab (Stuttg). 1976 Aug;29(8):188-92.
8
[Detection of esterase D and glyoxalase I in human hair roots using agarose gel thin-layer electrophoresis].[利用琼脂糖凝胶薄层电泳检测人发根中的酯酶D和乙二醛酶I]
Z Rechtsmed. 1983;90(1):15-8. doi: 10.1007/BF01886062.
9
[Determination of glyoxalase 1 groups in liquid and dried blood by polyacrylamide gel electrophoresis].[通过聚丙烯酰胺凝胶电泳法测定液体和干血中乙二醛酶1组]
Sud Med Ekspert. 1988 Jul-Sep;31(3):36-8.
10
Discussion of "The simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase".《乙二醛酶I、酯酶D和磷酸葡萄糖变位酶的同步分离》的讨论
J Forensic Sci. 1987 Nov;32(6):1498-500.
Zotero 插件