在一块丙烯酰胺凝胶中对八种红细胞酶进行表型分析。

Phenotyping of eight erythrocytic enzymes in one acrylamide gel.

作者信息

Lee C L, Ying R L

出版信息

Am J Clin Pathol. 1979 Jun;71(6):672-6. doi: 10.1093/ajcp/71.6.672.

DOI:10.1093/ajcp/71.6.672

Abstract

A procedure has been developed to phenotype eight erythrocytic enzymes, phosphoglucomutase (PGM1), adenylate kinase (AK), 6-phosphogluconate dehydrogenase (6PGD), adenosine deaminase (ADA), glyoxalase (GLO), esterase-D (EsD), acid phosphatase (AcP), and glutamic pyruvate transaminase (GPT) in one acrylamide gel and also to detect the presence of common abnormal hemoglobins. The agar overlay technic has been eliminated. This simplified procedure renders the phenotyping of erythrocytic enzymes practical in paternity testing.

摘要

已开发出一种方法，可在一块丙烯酰胺凝胶中对八种红细胞酶进行表型分析，这八种酶分别是磷酸葡萄糖变位酶（PGM1）、腺苷酸激酶（AK）、6-磷酸葡萄糖酸脱氢酶（6PGD）、腺苷脱氨酶（ADA）、乙二醛酶（GLO）、酯酶-D（EsD）、酸性磷酸酶（AcP）和谷丙转氨酶（GPT），同时还能检测常见异常血红蛋白的存在。琼脂覆盖技术已被摒弃。这种简化方法使红细胞酶的表型分析在亲子鉴定中切实可行。

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