Bussereau F, Flamand A
Ann Microbiol (Paris). 1978 Jul;129B(1):71-86.
Coinfection of cells with vesicular stomatitis virus (VSV) of Indiana and New-Jersey serotypes were performed. Thermosensitive mutants (ts) of VSV Indiana and the wild type strain (+) of New-Jersey were used. Harvests and titrations were made at permissive(PT) and nonpermissive (NPT) temperatures. It was shown that the harvest was mainly composed of one parental-like infectious particles. The dominance of one serotype over the other was shown to be a function of the relative multiplicity of the two viruses; the presence of a thermosensitive lesion imparts a disadvantage to the corresponding serotype. Non parental-like particles were also detected. As expected, these particles were detected only in two conditions. 1) Harvest performed at NPT and titrations allowed at PT.- Most of the infectious particles (i.e. twin particles) resistant to anti-Nj serum developped a plaque (i.e. mixed-plaque)containing virions of both serotypes: Indiana (ts) and New-Jersey (+). After sonication or EDTA treatment of the harvest, prior to titrations, no more mixed-plaques were formed. Examination of the harvest by electron microscopy showed that 7-17 % of the particles formed aggregates; therefore, it is likely that the twin-particles are in fact aggregates. 2) Harvest performed at PT and titrations allowed at NPT.-It has been shown that 1 % of the wild type infectious particles was resistant to anti-Nj serum even though being of Nj genotype. It was inactivated by a mixture of anti-Nj and anti-In sera and therfore behave as pseudotypes. But since twin particles, when plated at Nt, would give rise to an homogenous progeny from New-Jersey (+), they could be confused with pseudotypes. Under those conditions there is no absolute evidence that phenotypic mixing really occurs between VSV of Indiana and New-Jersey serotypes.
用印第安纳血清型和新泽西血清型的水疱性口炎病毒（VSV）对细胞进行共感染。使用了VSV印第安纳血清型的温度敏感突变体（ts）和新泽西血清型的野生型毒株（+）。在允许温度（PT）和非允许温度（NPT）下进行收获和滴定。结果表明，收获物主要由一种亲本样感染性颗粒组成。一种血清型对另一种血清型的优势显示为两种病毒相对复数的函数；温度敏感损伤的存在使相应血清型处于劣势。还检测到了非亲本样颗粒。正如预期的那样，这些颗粒仅在两种情况下被检测到。1）在NPT进行收获并在PT进行滴定。-大多数对抗Nj血清有抗性的感染性颗粒（即双颗粒）形成了一个含有印第安纳（ts）和新泽西（+）两种血清型病毒粒子的噬菌斑（即混合噬菌斑）。在滴定前对收获物进行超声处理或EDTA处理后，不再形成混合噬菌斑。通过电子显微镜检查收获物发现，7-17%的颗粒形成了聚集体；因此，双颗粒实际上很可能是聚集体。2）在PT进行收获并在NPT进行滴定。-已经表明，即使是Nj基因型，1%的野生型感染性颗粒也对抗Nj血清有抗性。它被抗Nj和抗In血清的混合物灭活，因此表现为假型。但是由于双颗粒在Nt接种时会产生来自新泽西（+）的同质后代，它们可能会与假型混淆。在这些条件下，没有绝对证据表明印第安纳血清型和新泽西血清型的VSV之间真的发生了表型混合。
