Martinez Isidoro, Barrera Jose C, Rodriguez Luis L, Wertz Gail W
Department of Microbiology, University of Alabama School of Medicine, Birmingham, AL 35294, USA.
Vaccine. 2004 Sep 28;22(29-30):4035-43. doi: 10.1016/j.vaccine.2004.03.065.
Vesicular stomatitis virus (VSV) is the most common cause of vesicular disease outbreaks in livestock throughout the Western Hemisphere. Two major serotypes, Indiana and New Jersey, cause epidemic disease in pigs, cattle, and horses. We generated recombinant viruses derived from the Indiana serotype genome that were engineered to contain and express: (1) a single copy of the glycoprotein gene from the Indiana serotype (VSIV-GI); (2) a single copy of the glycoprotein gene from the New Jersey serotype (VSIV-GNJ); or (3) two copies of the glycoprotein gene, one from each of the two major VSV serotypes (VSIV-GNJGI) [Martinez I, Rodriguez LL, Jimenez C, Pauszek SJ, Wertz GW. Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host. J Virol 2003;77(14):8039-47]. These recombinant viruses and a highly pathogenic New Jersey field isolate (VSNJV), from which the GNJ gene was derived, were inoculated into swine, a natural host, and the induction of neutralizing antibodies to both serotypes was analyzed. The neutralizing antibody response induced by VSIV-GI, VSIV-GNJ and VSNJV was serotype-specific, according to the glycoprotein expressed. VSIV-GNJGI expressed both glycoproteins stably through multiple rounds of replication in swine and induced neutralizing antibodies against both VSV serotypes, with a dominance of the Indiana serotype in the serological response. Pigs immunized with VSIV-GI or VSIV-GNJ were protected against homologous high dose virus challenge. Pigs inoculated with VSIV-GNJGI were protected against challenge with VSIV-GI but three of four animals developed lesions after challenge with the highly pathogenic New Jersey field isolate.
水泡性口炎病毒（VSV）是西半球家畜水泡性疾病爆发的最常见原因。两种主要血清型，印第安纳型和新泽西型，可在猪、牛和马中引发流行性疾病。我们构建了源自印第安纳血清型基因组的重组病毒，这些病毒经过改造后包含并表达：（1）印第安纳血清型糖蛋白基因的单拷贝（VSIV-GI）；（2）新泽西血清型糖蛋白基因的单拷贝（VSIV-GNJ）；或（3）糖蛋白基因的两个拷贝，分别来自两种主要VSV血清型中的每一种（VSIV-GNJGI）[马丁内斯I，罗德里格斯LL，希门尼斯C，波泽克SJ，韦茨GW。水泡性口炎病毒糖蛋白是自然宿主猪发病机制的决定因素。《病毒学杂志》2003年；77(14):8039 - 47]。将这些重组病毒以及从中获取GNJ基因的高致病性新泽西野外分离株（VSNJV）接种到自然宿主猪体内，并分析针对两种血清型的中和抗体的诱导情况。根据所表达的糖蛋白，VSIV-GI、VSIV-GNJ和VSNJV诱导的中和抗体反应具有血清型特异性。VSIV-GNJGI在猪体内经过多轮复制后稳定表达两种糖蛋白，并诱导产生针对两种VSV血清型的中和抗体，在血清学反应中印第安纳血清型占主导。用VSIV-GI或VSIV-GNJ免疫的猪对同源高剂量病毒攻击具有抵抗力。接种VSIV-GNJGI的猪对VSIV-GI攻击具有抵抗力，但四分之三的动物在受到高致病性新泽西野外分离株攻击后出现病变。
