Dresser D W
National Institute for Medical Research, London, U.K.
J Immunol Methods. 1990 May 8;129(1):1-8. doi: 10.1016/0022-1759(90)90413-p.
A method has been devised to make rapid assessments of plaque size simultaneously with plaque enumeration. The method depends on an inexpensive and simple interfacing of a weighted counter to a microcomputer. Comparisons made between relative (weighted counter) and absolute (photographic) assessments of plaque size, show that the relationship is not linear. It has been shown that assay conditions must be rigidly controlled for valid size comparisons to be made. IgM and IgG PFC specific for sheep RBC, burro RBC and TNP-, in primary and secondary, active and adoptive responses, were used in these experiments. An application of the method in antibody feedback experiments is described.
已设计出一种方法，可在菌斑计数的同时对菌斑大小进行快速评估。该方法依赖于将一个加权计数器与一台微型计算机进行廉价且简单的接口连接。对菌斑大小的相对（加权计数器）评估和绝对（摄影）评估之间的比较表明，两者关系并非线性。已证明，为了进行有效的大小比较，必须严格控制检测条件。在这些实验中，使用了在初次和二次、主动和过继反应中对绵羊红细胞、驴红细胞以及三硝基苯特异性的IgM和IgG空斑形成细胞。本文描述了该方法在抗体反馈实验中的应用。
