Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Histopathology. 2011 Apr;58(5):705-11. doi: 10.1111/j.1365-2559.2011.03789.x. Epub 2011 Mar 14.
The results of our earlier studies suggested that 14-3-3ε is involved in cancer cell survival and growth. However, it is not clear whether 14-3-3ε plays a role in tumour metastasis and patient outcome. The aim of this study was to determine whether 14-3-3ε is a marker for predicting hepatocellular carcinoma (HCC) metastasis and survival.
One hundred and fourteen patients with tissue-diagnosed primary HCC were followed for an average of 58.6 months. 14-3-3ε in liver tissues was analysed by immunohistochemistry, and quantified by a Quick score system. Correlation of 14-3-3ε with patient survival and metastasis was analysed with a Wilcoxon signed rank test, Kaplan-Meier survival curves, and Cox proportional hazard regression. Seventy-one of 114 patients (62.3%) had a significant increase of 14-3-3ε expression in HCC tissues, whereas normal tissues expressed weak or undetectable 14-3-3ε. Elevated 14-3-3ε expression was significantly associated with shortened overall survival and progression-free survival. Furthermore, 14-3-3ε overexpression increased the risk of metastasis 4.6-fold.
Overexpression of 14-3-3ε in primary HCC tissues predicts a high risk of extrahepatic metastasis and worse survival, and is a potential therapeutic target.
我们之前的研究结果表明，14-3-3ε 参与了癌细胞的存活和生长。然而，目前尚不清楚 14-3-3ε 是否在肿瘤转移和患者预后中发挥作用。本研究旨在确定 14-3-3ε 是否是预测肝细胞癌（HCC）转移和生存的标志物。
114 例经组织学诊断的原发性 HCC 患者接受了平均 58.6 个月的随访。采用免疫组织化学法分析肝组织中的 14-3-3ε，并通过快速评分系统进行定量。采用 Wilcoxon 符号秩检验、Kaplan-Meier 生存曲线和 Cox 比例风险回归分析 14-3-3ε 与患者生存和转移的相关性。114 例患者中有 71 例（62.3%）的 HCC 组织中 14-3-3ε 表达显著增加，而正常组织中 14-3-3ε 表达较弱或无法检测到。14-3-3ε 的高表达与总生存期和无进展生存期缩短显著相关。此外，14-3-3ε 过表达使转移风险增加 4.6 倍。
原发性 HCC 组织中 14-3-3ε 的过表达预示着肝外转移和生存不良的高风险，是一个潜在的治疗靶点。
