Scott Kathleen M, Fox Gordon, Girguis Peter R
Department of Integrative Biology, University of South Florida, Tampa, Florida, USA.
Methods Enzymol. 2011;494:281-99. doi: 10.1016/B978-0-12-385112-3.00014-7.
Physical, chemical, and biological processes commonly discriminate among stable isotopes. Therefore, the stable isotope compositions of biomass, growth substrates, and products often carry the isotopic fingerprints of the processes that shape them. Therefore, measuring isotope fractionation by enzymes and cultures of autotrophic microorganisms can provide insights at many levels, from metabolism to ecosystem function. Discussed here are considerations relevant to measuring isotope discrimination by enzymes as well as intact cells, with an emphasis on stable one-carbon isotopes and autotrophic microorganisms.
物理、化学和生物过程通常会区分稳定同位素。因此，生物质、生长底物和产物的稳定同位素组成常常带有塑造它们的过程的同位素指纹。所以，通过自养微生物的酶和培养物来测量同位素分馏可以在从代谢到生态系统功能的多个层面提供见解。本文讨论了与通过酶以及完整细胞测量同位素分馏相关的注意事项，重点是稳定的单碳同位素和自养微生物。
