Nakano A, Harada T, Morikawa S, Kato Y
Department of Medicine, Shimane Medical University, Izumo, Japan.
Acta Pathol Jpn. 1990 Feb;40(2):107-15. doi: 10.1111/j.1440-1827.1990.tb01549.x.
CD45 antigen (leukocyte common antigen), a unique and ubiquitous membrane glycoprotein with a molecular mass of about 200 kDa, is expressed on almost all hematopoietic cells except for mature erythrocytes. However, the biological function of this glycoprotein still remains to be resolved. In order to clarify the role of CD45 antigen in hematopoietic cell differentiation and function, its expression on human leukemia/lymphoma cell lines was studied by membrane immunofluorescence. Thirty-eight established cell lines were analyzed using T29/33, a monoclonal antibody (MoAb) that recognizes the common epitopes of this glycoprotein molecule. Conventional cell marker studies were also carried out on these cell lines to compare their CD45 expression. It was shown that CD45 expression varies among B-lineage cells depending on cell differentiation, in contrast to its stable expression on leukemic T cell (6/6, positive) and myeloid (5/5, positive) lineage cell lines. On the other hand, only two out of six histiomonocytoid lineage cell lines were positive. Human T cell leukemia/lymphoma virus type I (HTLV-I)-associated T cell lines derived from peripheral blood leukocytes of patients with adult T cell leukemia/lymphoma (ALT/L) in Japan did not express CD45 on their cell surface. Taken together, these observations suggest that CD45 has a functional role in hematopoietic cell activation and differentiation.
CD45抗原（白细胞共同抗原）是一种独特且普遍存在的膜糖蛋白，分子量约为200 kDa，几乎在除成熟红细胞外的所有造血细胞上均有表达。然而，这种糖蛋白的生物学功能仍有待阐明。为了明确CD45抗原在造血细胞分化和功能中的作用，通过膜免疫荧光法研究了其在人白血病/淋巴瘤细胞系上的表达。使用识别这种糖蛋白分子共同表位的单克隆抗体（MoAb）T29/33分析了38个已建立的细胞系。还对这些细胞系进行了常规细胞标志物研究，以比较它们的CD45表达。结果表明，与在白血病T细胞系（6/6，阳性）和髓系细胞系（5/5，阳性）上稳定表达不同，CD45在B系细胞中的表达因细胞分化而异。另一方面，六个组织单核细胞系细胞中只有两个呈阳性。源自日本成人T细胞白血病/淋巴瘤（ALT/L）患者外周血白细胞的人I型T细胞白血病/淋巴瘤病毒（HTLV-I）相关T细胞系在其细胞表面不表达CD45。综上所述，这些观察结果表明CD45在造血细胞激活和分化中具有功能作用。
