Leonardi Rosalia, Almeida Luis Eduardo, Rusu Mugurel, Sicurezza Edoardo, Palazzo Giuseppe, Loreto Carla
Department of Dentistry, Faculty of Dentistry, University of Catania, Policlinico Universitario, Catania, Italy.
J Craniofac Surg. 2011 Mar;22(2):504-8. doi: 10.1097/SCS.0b013e3182087394.
This study investigated if tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) immunohistochemical expression in human temporomandibular joint (TMJ)-degenerated disks correlates to the degree of tissue damage to elucidate the possible involvement of this apoptotic pathway in TMJ disk degeneration. Twenty-one TMJ displaced disk from 12 patients were affected by anterior disk displacement with reduction and 9 by anterior disk displacement without reduction processed immunohistochemically with TRAIL antibody. Histopathologic grading of the disk degeneration was carried out in each specimen. The mean histopathologic score of the TMJ degenerated disks was 4.77±1.26 (minimum, 2; maximum, 7). Immunolabeling for TRAIL was detected in the cytoplasm of the TMJ disk cells in every sample, although with different patterns of reactivity. The degree of TRAIL immunostaining was correlated to the histopathologic degeneration score obtained from the sample (Spearman ρ=0.617). Therefore, cell loss due to the involvement of TRAIL apoptotic pathway seems, in part, responsible for TMJ disk degeneration.
本研究调查了肿瘤坏死因子相关凋亡诱导配体（TRAIL）在人类颞下颌关节（TMJ）退变盘组织中的免疫组化表达是否与组织损伤程度相关，以阐明该凋亡途径在TMJ盘退变中可能发挥的作用。选取12例患者的21个TMJ移位盘，其中12个为可复性盘前移位，9个为不可复性盘前移位，用TRAIL抗体进行免疫组织化学处理。对每个标本进行盘退变的组织病理学分级。TMJ退变盘的平均组织病理学评分为4.77±1.26（最小值为2，最大值为7）。尽管反应模式不同，但在每个样本的TMJ盘细胞胞质中均检测到TRAIL免疫标记。TRAIL免疫染色程度与样本的组织病理学退变评分相关（Spearman ρ=0.617）。因此，TRAIL凋亡途径参与导致的细胞丢失似乎在一定程度上导致了TMJ盘退变。
