Shormanov V K, Baranov Iu N, Duritsyn E P, Maslov S V, Pronichenko E I, Ganiev S V
Sud Med Ekspert. 2010 Nov-Dec;53(6):39-41.
Optimal conditions for the extraction of bancol from the biological material with toluene are described. The possibility of its purification and separation from co-extracted compounds on a silicagel L column, 40/100 mcm is illustrated. Identification and quantitative determination of bancol isolated from the cadaverous liver were performed by the electron spectrophotometry technique, thin-layer chromatography, and high performance liquid chromatography using normal-phase sorbents. A method of bancol detection was adapted for the purpose of forensic medical expertise and applied for the postmortem examination of the cadaverous tissues.
描述了用甲苯从生物材料中提取班科尔的最佳条件。说明了在40/100微米硅胶L柱上从共提取化合物中纯化和分离班科尔的可能性。通过电子分光光度法、薄层色谱法和使用正相吸附剂的高效液相色谱法对从尸体肝脏中分离出的班科尔进行了鉴定和定量测定。为法医鉴定目的改编了一种班科尔检测方法,并将其应用于尸体组织的死后检查。