Takao Shinichi, Hara Michimaru, Okazaki Tomio, Suzuki Kazuo
Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute.
Kansenshogaku Zasshi. 2011 Jan;85(1):31-6. doi: 10.11150/kansenshogakuzasshi.85.31.
Luminex xTAG respiratory viral panel FAST (RVP FAST) assay detects 17 human respiratory virus strains per measurement. Studying RVP FAST efficacy in detecting respiratory viruses in 67 aspirate samples from the nasal cavities of children with acute respiratory infection, we compared RVP FAST results to those of conventional nucleic acid amplification tests (NAT), e.g., real-time PCR, targeting 8 strains. RVP FAST assay detected 13 strains (98 isolates) in 59 of 67 samples. Of these, 8--influenza virus (Inf.V)-AH1, Inf. V-AH3, novel Inf.V-AH1, and Inf.V-B, and adenovirus, RS virus, metapneumovirus, and bocavirus--were compared to NAT results. RVP FAST showed higher sensitivity (83.3-100%) and specificity (98.2-100%) than NAT. RVP FAST also detected coronavirus (CoV) 229E, OC43, NL63, and HKU1 from 10 virus strain samples and enterovirus and/or rhinovirus from 35. RVP FAST assay thus comprehensively detects clinically important viruses in a single measurement, making RVP FAST assay useful in detecting causative respiratory tract viruses.
Luminex xTAG呼吸道病毒快速检测板（RVP FAST）检测法每次检测可检测17种人类呼吸道病毒株。在研究RVP FAST检测急性呼吸道感染儿童鼻腔吸出物样本中呼吸道病毒的效能时，我们将RVP FAST的检测结果与传统核酸扩增检测（NAT）（例如针对8种病毒株的实时PCR）的结果进行了比较。RVP FAST检测法在67份样本中的59份中检测到了13种病毒株（98个分离株）。其中，8种病毒——甲型流感病毒（Inf.V）-AH1、甲型流感病毒-AH3、新型甲型流感病毒-AH1、乙型流感病毒，以及腺病毒、呼吸道合胞病毒、偏肺病毒和博卡病毒——与NAT结果进行了比较。RVP FAST显示出比NAT更高的灵敏度（83.3%-100%）和特异性（98.2%-100%）。RVP FAST还从10个病毒株样本中检测到了冠状病毒（CoV）229E、OC43、NL63和HKU1，并从35个样本中检测到了肠道病毒和/或鼻病毒。因此，RVP FAST检测法可在单次检测中全面检测临床上重要的病毒，使得RVP FAST检测法在检测引起呼吸道感染的病毒方面很有用。
