Department of Virology, Haartman Institute, University of Helsinki, FIN-00014 Helsinki, Finland.
J Virol Methods. 2012 Jun;182(1-2):82-6. doi: 10.1016/j.jviromet.2012.03.015. Epub 2012 Mar 23.
The aim of the study was to develop a real-time RT-PCR for the detection of enteroviruses (EVs) and rhinoviruses (RVs) and to assess the performance of the xTAG RVP Fast assay in comparison to a direct fluorescent assay (DFA), a real-time RT-PCR assay for the detection of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), and the EV/RV RT-PCR assay developed in this study. The performance of the RVP Fast assay was assessed in the analysis of 373 nasopharyngeal samples. For the viruses of the DFA panel, detection rates of 27.6% and 23.8% were obtained by RVP and DFA, respectively, in analysis of a set of 297 samples collected in 2009-2010. These results show statistically significant superiority of the RVP Fast assay (P=0.049). For RSV, hMPV, EV, and RV, detection rates of 48.0% and 45.2% were achieved by RVP and RT-PCR, respectively. For individual targets, increased detection of EV/RV (P=0.043) and decreased detection of influenza A virus (P=0.004) by RVP in comparison to real-time RT-PCR was observed. The results of the present study imply the need to adjust the InfA component of the RVP Fast assay to also cover the InfA(H1N1) 2009 virus.
本研究的目的是开发一种用于检测肠道病毒（EVs）和鼻病毒（RVs）的实时 RT-PCR，并评估 xTAG RVP Fast 检测试剂盒与直接荧光检测法（DFA）、用于检测呼吸道合胞病毒（RSV）和人偏肺病毒（hMPV）的实时 RT-PCR 检测法以及本研究中开发的 EV/RV RT-PCR 检测法的性能。该 RVP Fast 检测试剂盒的性能通过对 373 个鼻咽样本的分析进行评估。对于 DFA 检测试剂盒组中的病毒，在对 2009-2010 年收集的 297 个样本进行分析时，RVP 和 DFA 的检测率分别为 27.6%和 23.8%。这些结果表明 RVP Fast 检测试剂盒具有统计学上的优越性（P=0.049）。对于 RSV、hMPV、EV 和 RV，RVP 和 RT-PCR 的检测率分别为 48.0%和 45.2%。对于个别靶标，与 RT-PCR 相比，RVP 对 EV/RV 的检测率增加（P=0.043），对流感 A 病毒的检测率降低（P=0.004）。本研究的结果表明，需要调整 RVP Fast 检测试剂盒中的 InfA 组件，以涵盖 InfA(H1N1) 2009 病毒。
