Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
J Econ Entomol. 2011 Feb;104(1):125-36. doi: 10.1603/ec10151.
Laboratory and field studies were undertaken to determine the effects of increased numbers of trichomes on seedling stems, petioles, and first true leaves of Brassica napus L., canola, on the feeding and behavior of the crucifer flea beetle Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae). Seedlings of 'Westar' canola with genes inserted from Arabidopsis thaliana L. for increased trichome production, called Hairyl, were tested against Westar seedlings in no-choice and choice laboratory tests, and against parental plants and other cultivars grown from seed with and without insecticide in field trials at Saskatoon and Lethbridge, Canada. Analyses ofprefeeding and feeding behavior in no-choice tests of first true leaves found that flea beetles interacted with their host while off Hairyl leaves more so than beetles presented with leaves of Westar. Beetles required twice as much time to reach satiation when feeding on leaves with increased pubescence than on Westar leaves. In laboratory choice tests, flea beetles fed more on cotyledons and second true leaves of Westar than on comparable tissues of the transgenic line. In field trials, variations in feeding patterns were seen over time on cotyledons of the line with elevated trichomes. However, all four young true leaves of Hairyl seedlings were fed upon less than were the parental lines. Feeding on Hairyl plants frequently occurred at levels equal to or less than on cultivars grown from insecticide-treated seed. This study highlights the first host plant resistance trait developed in canola, dense pubescence, with a strong potential to deter feeding by crucifer flea beetles.
实验室和田间研究旨在确定增加油菜（ Brassica napus L.）幼苗茎、叶柄和第一片真叶上的毛状体数量对十字花科跳蚤甲虫（ Phyllotreta cruciferae ）（鞘翅目：叶甲科）取食和行为的影响。对来自拟南芥（ Arabidopsis thaliana ）的基因插入物以增加毛状体产生的“Westar”油菜（称为 Hairyl）的幼苗进行了无选择和选择实验室测试，以及在萨斯卡通和莱斯布里奇（加拿大）的田间试验中对亲本植物和其他从有和无杀虫剂种子种植的品种进行了测试。在无选择的第一片真叶预食和取食行为分析中发现，跳蚤甲虫在离开 Hairyl 叶片时与宿主相互作用的程度比接触 Westar 叶片时更为强烈。当以增加的茸毛叶片为食时，甲虫达到饱食所需的时间是喂食 Westar 叶片的两倍。在实验室选择测试中，跳蚤甲虫在 Westar 的子叶和第二片真叶上的取食量大于在转基因系的可比组织上的取食量。在田间试验中，随着时间的推移，在毛状体升高的系的子叶上观察到了取食模式的变化。然而，Hairyl 幼苗的所有四片真叶的取食量都少于亲本系。对 Hairyl 植株的取食经常发生在与从经杀虫剂处理的种子种植的品种相同或更低的水平。本研究强调了油菜中第一个被开发的宿主植物抗性性状，即浓密的茸毛，具有强烈阻止十字花科跳蚤甲虫取食的潜力。
