Faculty of Pharmacy and Biochemistry Zagreb, University of Zagreb, Croatia.
Acta Pharm. 2011 Mar;61(1):93-102. doi: 10.2478/v10007-011-008-4.
A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1 HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.
提出了一种新的、简单的火焰原子吸收光谱法（FAAS），用于同时测定儿童血清中的铁和铜。该方法基于单一的样品预处理步骤（用 3 mol/L HCl 进行蛋白沉淀，比例为 1:1）和使用 1.5 mol/L HCl 标准溶液进行单步校准。在方法优化过程中使用了短时间的多因素设计实验。该方法具有与参考方法相当的准确性、灵敏度和精密度。该新方法简单、省时、省力、省血清，特别是在儿科诊断中非常重要。
