Department of Clinical Immunology, Section for Transfusion Medicine, Capital Region Blood Bank, Rigshospitalet 2034, University of Copenhagen, Denmark.
Clin Appl Thromb Hemost. 2011 Nov-Dec;17(6):E211-7. doi: 10.1177/1076029610397183. Epub 2011 Mar 14.
The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10(9)/L (all P < .03), except for heparin-TRAP (50 × 10(9)/L, P = .008) and citrate-ASPI (150 × 10(9)/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10(9)/L (P < .05). In conclusion, platelet concentration of <150 × 10(9)/L may influence Multiplate which should be considered in clinical settings. Furthermore, the findings emphasize the importance of evaluating haemostasis in its natural matrix, WB.
全血（WB）血小板功能检测的 Multiplate 已显示出良好的效果，可以识别出接受抗血小板治疗且存在血栓再形成风险增加的患者。在本研究中，分析了低血小板计数对血小板聚集的影响，并与人工基质富血小板血浆（PRP）中的聚集结果进行了比较。肝素化和柠檬酸盐抗凝的血液用自体血浆稀释至 WB 样本中的血小板浓度为 200 至 25×10(9)/L（n=10）和 PRP 样本中的 200 至 100×10(9)/L（n=7）。通过 ADP、ASPI、COL 和 TRAP 试验研究血小板聚集。当血小板浓度≤100×10(9)/L 时，WB 反应均降低（均 P <.03），肝素-TRAP（50×10(9)/L，P =.008）和柠檬酸盐-ASPI（150×10(9)/L，P =.03）除外。一般来说，在 200 至 100×10(9)/L 的血小板浓度下，WB 样本比 PRP 样本显示出更高的聚集性（P <.05）。总之，血小板浓度<150×10(9)/L 可能会影响 Multiplate，在临床环境中应考虑到这一点。此外，这些发现强调了在其自然基质 WB 中评估止血的重要性。
