Department of Pathology, Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, UK.
Eur Respir J. 2011 Oct;38(4):911-7. doi: 10.1183/09031936.00176410. Epub 2011 Mar 15.
Endoscopic ultrasound-guided transbronchial or transoesophageal lymph node aspiration is increasingly used as a method of diagnosing nonsmall cell carcinoma. Data validating the accuracy of cell typing of nonsmall cell carcinoma using these cytological samples has not been assessed. 23 samples were identified in Edinburgh, UK and a further 25 in Cambridge, UK, with matching histological samples. The morphological cell type, as assessed on the cytological preparations and cell blocks, was recorded and immunohistochemical staining was performed, where possible, as an adjunct. The final cell type, as assessed by morphology with or without immunohistochemistry, was correlated with that reported in the paired histological samples. Cell blocks with tumour were available in 39 out of 48 cases. The accuracy of cell typing when no cell block was available was four out of nine cases. This increased to 25 out of 39 when a cell block was available, increasing to 33 out of 39 with the addition of immunohistochemistry. The overall accuracy of classification was 37 out of 48 cases. Accurate cell typing of nonsmall cell carcinomas can be performed using endoscopically derived fine-needle aspirates. The importance of obtaining sufficient material for the production of cell blocks is critical in allowing optimal assessment.
经内镜超声引导经支气管或经食管淋巴结抽吸术越来越多地被用作诊断非小细胞癌的一种方法。尚未评估使用这些细胞学样本对非小细胞癌进行细胞分型的准确性数据。在英国爱丁堡和剑桥分别确定了 23 个样本和另外 25 个样本，并与匹配的组织学样本进行了比较。记录了在细胞学标本和细胞块上评估的形态学细胞类型，并尽可能进行免疫组织化学染色作为辅助。通过形态学检查（包括或不包括免疫组织化学检查）评估的最终细胞类型与配对组织学样本中报告的细胞类型相关。在 48 例中有 39 例可获得带有肿瘤的细胞块。在没有细胞块的情况下进行细胞分型的准确性为 9 例中有 4 例。当有细胞块时，这一比例增加到 39 例中有 25 例，当加入免疫组织化学检查时，这一比例增加到 39 例中有 33 例。总的分类准确性为 48 例中有 37 例。通过内镜获得的细针抽吸物可以对非小细胞癌进行准确的细胞分型。获得足够的细胞块材料以进行最佳评估对于获得准确的细胞分型至关重要。
