Sujita K, Okuno F, Tanaka Y, Hirano Y, Inamoto Y, Eto S, Arai M
First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Biochem Biophys Res Commun. 1990 May 16;168(3):1217-22. doi: 10.1016/0006-291x(90)91158-o.
In the present study, the expression of IL-1 receptor (IL-1 R) on the primary cultured hepatocytes was determined and the effect of IL-1 on the intracellular amount of cytochrome P-450 was investigated. The kinetics of IL-1R on the hepatocytes was studied by [125I]-IL-1 alpha and revealed that 5,000 molecules of IL-1 bound to a hepatocyte with a Kd of 4.0 x 10(-9) M. Incubation of the hepatocytes with IL-1 for 18 hrs decreased the contents of cytochrome P-450 in a dose-dependent manner. These findings suggest that IL-1 potentially depresses the drug metabolism involving IL-1R which is expressed on hepatocytes.
在本研究中，测定了原代培养肝细胞上白细胞介素-1受体（IL-1R）的表达，并研究了白细胞介素-1对细胞色素P-450细胞内含量的影响。通过[125I]-IL-1α研究了肝细胞上IL-1R的动力学，结果显示5000个IL-1分子以4.0×10（-9）M的解离常数与一个肝细胞结合。用IL-1孵育肝细胞18小时，细胞色素P-450的含量呈剂量依赖性降低。这些发现表明，IL-1可能通过肝细胞上表达的IL-1R抑制药物代谢。
