Hoffman R W, Rettenmaier L J, Takeda Y, Hewett J E, Pettersson I, Nyman U, Luger A M, Sharp G C
Veterans Administration Research Service, Harry S Truman Memorial Veterans Hospital, Columbia, Missouri 65201.
Arthritis Rheum. 1990 May;33(5):666-73. doi: 10.1002/art.1780330509.
Serum samples from patients with connective tissue disease (CTD) were characterized using a recently developed enzyme-linked immunosorbent assay for reactivity with individual specific polypeptides of U1 small nuclear ribonucleoproteins, including the U1-70-kd protein. The distribution of HLA antigens was compared in CTD patients with and in those without anti-U1-70-kd autoantibodies and in normal controls. The frequencies of HLA-DR4 and HLA-DRw53 were increased among the anti-U1-70-kd autoantibody positive CTD group compared with the frequencies in anti-U1-70-kd autoantibody negative systemic lupus erythematosus patients and compared with normal controls. We conclude that the presence of autoantibodies reactive with the anti-U1-70-kd protein antigen is associated with HLA-DR4 and HLA-DRw53.
利用最近开发的一种酶联免疫吸附测定法，对结缔组织病（CTD）患者的血清样本进行了表征，以检测其与U1小核核糖核蛋白的各个特定多肽（包括U1 - 70kd蛋白）的反应性。比较了有和没有抗U1 - 70kd自身抗体的CTD患者以及正常对照中HLA抗原的分布情况。与抗U1 - 70kd自身抗体阴性的系统性红斑狼疮患者及正常对照相比，抗U1 - 70kd自身抗体阳性的CTD组中HLA - DR4和HLA - DRw53的频率有所增加。我们得出结论，与抗U1 - 70kd蛋白抗原反应的自身抗体的存在与HLA - DR4和HLA - DRw53相关。
