Lin H
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1990 Feb;12(1):19-24.
Human alpha-ANP was synthesised using a yeast system. In order to obtain correct expression of the human alpha-ANP gene, a few bases on one strand were substituted by the corresponding yeast sequence, but those bases were not replaced in the complementary so a few unpaired bases existed. The gene was cloned into a shuttle vector (with alpha-factor). The recombinants containing different human alpha-ANP DNA sequences were separated by BglII restriction analysis. We compared the expression level of human alpha-ANP from two kinds of genes and the results showed that the ANP expression level by the gene containing yeast sequences was lower (0.5-0.7 mg/L) than that expressed by the original gene (0.8-1.0 mg/L).
人α-心钠素是利用酵母系统合成的。为了获得人α-心钠素基因的正确表达，一条链上的几个碱基被相应的酵母序列取代，但互补链上的这些碱基未被替换，因此存在一些未配对的碱基。该基因被克隆到一个穿梭载体（带有α-因子）中。通过BglII限制性分析分离出含有不同人α-心钠素DNA序列的重组体。我们比较了两种基因的人α-心钠素表达水平，结果表明，含有酵母序列的基因的ANP表达水平（0.5 - 0.7毫克/升）低于原始基因表达的水平（0.8 - 1.0毫克/升）。
