State Key Laboratory of Crop Biology, College of Horticulture, Shandong Agricultural University, 271018 Tai'an, People's Republic of China.
Protein J. 2011 Mar;30(3):194-200. doi: 10.1007/s10930-011-9320-6.
Fruit bagging is a very effective method for study of fruit qualities and anthocyanin synthesis. The characterization of differentially expressed proteins that were isolated from both bagged and normal fruit skin tissue is apparently an essential parameter for understanding the effect of shading on fruit qualities and to understand the mechanism of fruit coloring in Pyrus communis. Proteome maps of both bagged and normal P. communis 'Placer' fruit skin were obtained by performing two-dimensional electrophoresis analysis and compared to assess the extent to which protein distribution differed in pear skin. The comparative analysis showed 38 differentially expressed proteins between the two samples: with three protein spots up-regulated and 35 down-regulated in the bagged fruit. Differentially expressed protein spots were subjected to matrix-assisted laser desorption ionization time of flight (MALDI-TOF) analysis and the data compared to that of known proteins to deduce their possible functions. Of these, 21 protein spots were identified and classified into functional classes. These identified proteins were mainly involved in photosynthesis, signal transduction, energy pathway, protein folding and assembly, and carbohydrate and acidity metabolisms, and were under-expressed in bagged fruit skins. This work provides a first characterization of the proteome changes in response to fruit bagging treatment in red pears.
果实套袋是研究果实品质和花色苷合成的一种非常有效的方法。从套袋和正常果实果皮组织中分离的差异表达蛋白的特性分析显然是理解遮光对果实品质的影响以及理解梨果实着色机制的一个重要参数。通过二维电泳分析获得了套袋和正常‘Placer’梨果实果皮的蛋白质组图谱，以评估梨果皮中蛋白质分布的差异程度。比较分析显示，两个样本之间有 38 个差异表达蛋白：套袋果实中有 3 个蛋白点上调，35 个蛋白点下调。对差异表达蛋白点进行基质辅助激光解吸电离飞行时间（MALDI-TOF）分析，并将数据与已知蛋白进行比较，以推断其可能的功能。其中，21 个蛋白点被鉴定，并分为功能类别。这些鉴定的蛋白主要参与光合作用、信号转导、能量途径、蛋白折叠和组装以及碳水化合物和酸度代谢，在套袋果实皮中表达下调。这项工作首次对红梨果实套袋处理的蛋白质组变化进行了描述。
