Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Bioresour Technol. 2011 May;102(10):5815-23. doi: 10.1016/j.biortech.2011.02.064. Epub 2011 Feb 24.
Lipid accumulation abilities of two endophytic fungal isolates - Colletotrichum sp. and Alternaria sp. grown under optimum and nutrient-stress conditions were investigated and compared. Significant variations in lipid contents, ranging from 30% to 58% of their dry biomass were found in liquid culture using various carbon sources. Since, >50% of the total lipid was estimated to be neutral lipid for both the fungal species, predicted biodiesel properties were theoretically calculated based upon the determined fatty acid profiles; and the values were found to be comparable to those of commonly used plant oils for biodiesel production. The two endophytes grew successfully on the combined rice straw and wheat bran as substrate that was degraded by their secretory enzymes including cellulase [1.21-2.51 FPU/g dry substrate (gds)] in solid state fermentation and produced substantial amount of lipid (60.32-84.30 mg/gds). Our study highlights the potential utilities of these two novel endophytic fungi as biodiesel feedstock.
研究并比较了两种内生真菌（炭疽菌和链格孢菌）在最佳和营养胁迫条件下的脂类积累能力。在使用各种碳源的液体培养中，发现其干生物量的脂质含量有显著变化，范围从 30%到 58%。由于两种真菌的总脂质中估计有>50%为中性脂质，因此根据测定的脂肪酸谱预测了生物柴油特性；并且发现这些值与常用于生物柴油生产的植物油脂相当。这两种内生真菌在水稻秸秆和麦麸的混合物上成功生长，这些混合物被它们的分泌酶（包括纤维素酶[1.21-2.51 FPU/g 干基质（gds）]）降解，并在固态发酵中产生大量的脂质（60.32-84.30 mg/gds）。我们的研究强调了这两种新型内生真菌作为生物柴油原料的潜在用途。
