固态发酵中米曲霉 A-4 纤维素酶对小麦秸秆的直接微生物转化为油脂。
Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation.
机构信息
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China.
出版信息
Bioresour Technol. 2010 Oct;101(19):7556-62. doi: 10.1016/j.biortech.2010.04.027. Epub 2010 May 4.
Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation (SSF) was investigated. In submerged fermentation, A. oryzae A-4 accumulated lipid to 15-18.15% of biomass when pure cellulose was utilized as the sole substrate. In SSF of the wheat straw and bran mixture, A. oryzae A-4 yielded lipid of 36.6mg/g dry substrate (gds), and a cellulase activity of 1.82 FPU/gds with 25.25% of holocellulose utilization in the substrates were detected on the 6th day. The lipid yield reached 62.87 mg/gds in SSF on the 6th day under the optimized conditions from Plackett-Burman design (PBD). Cellulase secretion of A. oryzae A-4 was found to influence the lipid yield. Dilute acid pretreatment of the straw and addition of some agro-industrial wastes to the straw could enhance lipid production of A. oryzae A-4.
以固态发酵（SSF）的方式，利用米曲霉 A-4 这一纤维素分解真菌，直接将小麦秸秆转化为微生物油脂。在液体发酵中，当纯纤维素作为唯一的基质时，米曲霉 A-4 可将生物量的 15-18.15%转化为油脂。在小麦秸秆和麸皮混合物的 SSF 中，米曲霉 A-4 产生的油脂量为 36.6mg/g 干基质（gds），纤维素酶活为 1.82 FPU/gds，基质中全纤维素的利用率为 25.25%，在第 6 天即可检测到。通过 Plackett-Burman 设计（PBD）的优化条件，在第 6 天的 SSF 中，油脂的产量达到了 62.87mg/gds。米曲霉 A-4 的纤维素酶分泌会影响油脂的产量。对秸秆进行稀酸预处理，并向秸秆中添加一些农业工业废物，可以提高米曲霉 A-4 的油脂产量。
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