具有可调 pKa 和扩展动态范围的双荧光团比率型 pH 纳米传感器。
Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range.
机构信息
Laboratory of Biophysics and Surface Analysis, School of Pharmacy, Boots Sciences Building, University Park, Nottingham, UK NG7 2RD.
出版信息
Analyst. 2011 May 7;136(9):1799-801. doi: 10.1039/c1an15042a. Epub 2011 Mar 18.
Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green(®) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.
通过将两种 pH 敏感荧光团（异硫氰酸荧光素葡聚糖（FITC-D）和 Oregon Green（®）葡聚糖（OG-D））和一种参比荧光团（5-(和-6)-羧基四甲基罗丹明葡聚糖（TAMRA-D））组合包埋在生物相容性聚合物基质中，制备了具有可调 pK(a)的比率型 pH 纳米传感器。双荧光团 pH 纳米传感器允许测量从 pH 4.0 到 7.5 的扩展动态范围。
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