Larsson A, Jonsson L, Sjöquist J
Department of Medical and Physiological Chemistry, Uppsala University, Sweden.
ORL J Otorhinolaryngol Relat Spec. 1990;52(3):164-7. doi: 10.1159/000276127.
Serum levels of C4-containing circulating immune complexes in 48 patients with Bell's palsy were studied by an enzyme-linked immunosorbent assay (ELISA) using chicken antibodies. We have previously reported elevated levels of C1q- and C3-containing immune complexes when compared to the serum of 42 healthy persons. We now found the levels of C4-containing immune complexes in these sera to be slightly decreased, but these patients' sera had a slightly increased ability to activate and bind C4 to a model immune complex. The results indicate that the fairly low levels of C4-containing immune complexes were not due to a defect in C4 activation but point to a rapid modification of C4 in the immune complex in vivo or low binding of C4 to the immune complexes. This study also shows that it is preferable to use assays that detect C1q- and C3-containing immune complexes when analyzing sera from patients with Bell's palsy.
采用鸡抗体的酶联免疫吸附测定法（ELISA），对48例贝尔麻痹患者血清中含C4的循环免疫复合物水平进行了研究。我们之前曾报道，与42名健康人的血清相比，含C1q和C3的免疫复合物水平有所升高。我们现在发现，这些血清中含C4的免疫复合物水平略有下降，但这些患者的血清激活并将C4结合至模型免疫复合物的能力略有增强。结果表明，含C4的免疫复合物水平相当低并非由于C4激活缺陷，而是表明体内免疫复合物中C4发生了快速修饰，或者C4与免疫复合物的结合较弱。本研究还表明，在分析贝尔麻痹患者的血清时，最好使用检测含C1q和C3免疫复合物的检测方法。
