采用鸡抗人C3和抗人C1q微酶联免疫吸附测定法检测循环免疫复合物。

Determination of circulating immune complexes by chicken anti-human C3 and anti-human C1q microELISA.

作者信息

Larsson A, Jonsson L, Sjöquist J

机构信息

Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala, Sweden.

出版信息

J Immunol Methods. 1988 Oct 4;113(1):93-9. doi: 10.1016/0022-1759(88)90385-7.

DOI:10.1016/0022-1759(88)90385-7

PMID:3262687

Abstract

The present study reports on an anti-human C1q and an anti-human C3 microELISA for measuring circulating immune complexes (CIC). Affinity-purified chicken anti-human C3 and anti-human C1q were used as capture antibodies and protein A-alkaline phosphatase conjugate for detection. Chicken antibodies do not activate mammalian complement, do not react with rheumatoid factor and are not bound to anti-mammalian IgG antibodies or protein A, which often are used for detection in ELISA. They are therefore suitable as capture antibodies in CIC assays. We have tested Bell's palsy patients and found an increase in both anti-C3- and anti-C1q-containing CIC in acute and convalescent sera compared with the normal.

摘要

本研究报道了一种用于检测循环免疫复合物（CIC）的抗人C1q和抗人C3微量酶联免疫吸附测定法。亲和纯化的鸡抗人C3和抗人C1q用作捕获抗体，蛋白A-碱性磷酸酶偶联物用于检测。鸡抗体不会激活哺乳动物补体，不与类风湿因子反应，也不与常用于酶联免疫吸附测定检测的抗哺乳动物IgG抗体或蛋白A结合。因此，它们适合作为CIC测定中的捕获抗体。我们对贝尔麻痹患者进行了检测，发现与正常人相比，急性和恢复期血清中含抗C3和抗C1q的CIC均增加。