Wang Chun-yuan, Feng Shi-qing, Liu Yang, Wang Han-jie, Liang Xiao-fei, Li Hui, Han Ming-yuan, Yu Tie-qiang
Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin 300052, China.
Zhonghua Yi Xue Za Zhi. 2011 Jan 18;91(3):193-7.
To evaluate the ability of a kind of novel magnetic liposomes modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) to cross the blood spinal cord barrier (BSCB) so as to demonstrate whether or not they can accumulate at the lesions of injured spinal cord.
The novel liposomes were made through reverse-phase evaporation method modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) with an iron core. Thirty-six Wistar rats subject to spinal cord injury (SCI) at T10 were randomly divided into three groups (Groups I, II and III). The rats of Group III were injected with TAT-PEG loaded magnetic liposomes (4.55 mg/kg). The rats of GroupII received an injection of the equivalent PEG loaded magnetic liposomes while those of control group (GroupI) the equivalent normal saline. The accumulation of liposomes was observed by MRI (magnetic resonance imaging), Prussian blue staining, electron microscope and flame atomic absorption spectrophotometer.
This kind of TAT-PEG loaded magnetic liposomes could cross the BSCB and enter into the cells around the injured tissue. A low signal of T2WI on MRI could also be found in Group III. The results of flame atomic absorption spectrophotometer showed that the iron content accumulated around the lesion site in Group III was obviously higher than the other two groups (P < 0.05).
The TAT-PEG loaded magnetic liposomes may be employed as one kind of novel drug carrier to cross the BSCB and accumulate at tissue cells of spinal cord. It is likely to become a new therapy for SCI.
评估一种经聚乙二醇（PEG）和反式激活转录蛋白（TAT）修饰的新型磁性脂质体穿越血脊髓屏障（BSCB）的能力，以证明它们是否能在脊髓损伤部位蓄积。
采用反相蒸发法制备经聚乙二醇（PEG）和反式激活转录蛋白（TAT）修饰且含铁芯的新型脂质体。将36只T10脊髓损伤（SCI）的Wistar大鼠随机分为三组（I组、II组和III组）。III组大鼠注射载有TAT-PEG的磁性脂质体（4.55mg/kg）。II组大鼠注射等量载有PEG的磁性脂质体，而对照组（I组）注射等量生理盐水。通过磁共振成像（MRI）、普鲁士蓝染色、电子显微镜和火焰原子吸收分光光度计观察脂质体的蓄积情况。
这种载有TAT-PEG的磁性脂质体能够穿越血脊髓屏障并进入损伤组织周围的细胞。III组在MRI的T2WI上也可发现低信号。火焰原子吸收分光光度计的结果显示，III组损伤部位周围蓄积的铁含量明显高于其他两组（P<0.05）。
载有TAT-PEG的磁性脂质体可作为一种新型药物载体穿越血脊髓屏障并蓄积于脊髓组织细胞。它有可能成为脊髓损伤的一种新疗法。
