Department of Orthopaedics, Tianjin Medical University General Hospital, Anshan Road 154, Tianjin, PR China.
J Drug Target. 2010 Jul;18(6):420-9. doi: 10.3109/10611860903434001.
The blood-spinal cord barrier (BSCB) prevents many macromolecular agents from passing through to reach sites of injury in the spinal cord. This study evaluated the ability of a novel multifunctional liposome modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) containing an iron core to cross the BSCB using a rat model of spinal cord injury. Rats were examined daily for a period of three days after spinal cord injury and injection of either the multifunctional modified liposome or control formulations using a 3.0 T magnetic resonance imaging spectrometer. A low signal was observed in the T2-weighted images. Prussian blue staining and flame atomic absorption spectrophotometry revealed that significantly more iron accumulated around the lesion site in the experimental group than the control groups (P < 0.05). The findings from this study suggest that this multifunctional liposome carrier can cross the BSCB to accumulate around the lesion site.
血-脊髓屏障（BSCB）阻止许多大分子药物通过到达脊髓损伤部位。本研究使用大鼠脊髓损伤模型评估了一种新型多功能脂质体的能力，该脂质体通过与含有铁核心的聚乙二醇（PEG）和转染转导蛋白（TAT）修饰来穿过 BSCB。在脊髓损伤和注射多功能修饰脂质体或对照制剂后，大鼠每天接受检查，持续三天，使用 3.0T 磁共振成像光谱仪。在 T2 加权图像中观察到低信号。普鲁士蓝染色和火焰原子吸收分光光度法显示，实验组在损伤部位周围积累的铁明显多于对照组（P<0.05）。这项研究的结果表明，这种多功能脂质体载体可以穿过 BSCB 并在损伤部位周围积累。
