Department of Microbiology, Complexo Hospitalario Universitario de Vigo, Vigo, Spain.
J Virol Methods. 2011 May;173(2):357-63. doi: 10.1016/j.jviromet.2011.03.010. Epub 2011 Mar 16.
It has been suggested that some E6 human papillomavirus (HPV) type 16 variants could be involved in viral persistence and progression of HPV infection. A novel one-step allelic discrimination real-time PCR was evaluated for E6-350G variant detection in 102 endocervical HPV 16 positive samples. This assay was also used to assess the distribution of this variant in Spanish women with cervical cancer related to HPV 16. The detection limit for the allelic discrimination assay was 50 copies per reaction, even where the E6-350G variant represents only 20% of the variants in the sample. Complete concordance was observed between DNA sequencing and the novel AD RT-PCR assay. Fourteen E6-350T reference strains and 18 E6-350G variants were detected out of 32 endocervical samples from women with cervical cancer. The average age of women who were infected by the E6-350G HPV 16 variant was 10 years lower in these samples than in women who were infected by the reference strain. This novel allelic discrimination assay is a fast, sensitive and specific method for detection of the E6-350G HPV 16 variant.
有人提出，某些人乳头瘤病毒（HPV）16 型的 E6 变种可能与 HPV 感染的病毒持续存在和进展有关。本文评估了一种新型一步等位基因鉴别实时 PCR 法，用于检测 102 例宫颈 HPV16 阳性样本中的 E6-350G 变异。该检测还用于评估与 HPV16 相关的宫颈癌西班牙妇女中该变异的分布。等位基因鉴别检测的检测限为每个反应 50 个拷贝，即使 E6-350G 变异仅占样本中变异的 20%。DNA 测序和新型 AD RT-PCR 检测完全一致。在来自宫颈癌妇女的 32 例宫颈样本中，检测到 14 株 E6-350T 参考株和 18 株 E6-350G 变异株。在这些样本中，感染 E6-350G HPV16 变异株的妇女的平均年龄比感染参考株的妇女年轻 10 岁。这种新型等位基因鉴别检测是一种快速、敏感和特异的检测 E6-350G HPV16 变异的方法。
