Donawho C, Kripke M L
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Cancer Commun. 1990;2(3):101-7. doi: 10.3727/095535490820874597.
Non-melanoma skin cancers induced in mice by chemical carcinogens or ultraviolet radiation are often antigenic but rarely induce cross-protective immunity when tested by in vivo transplantation methods. We wished to determine whether melanocytic skin tumors behave similarly or whether they exhibit cross-reactive antigens in vivo. Three melanomas induced in C3H/HeNCr(MTV-) mice by initiation with ultraviolet radiation and promotion with croton oil or initiation with 7,12-dimethyl-benz[a]anthracene and promotion with 12-O-tetradecanoyl-phorbol-13-acetate or croton oil plus ultraviolet radiation were tested for immunogenicity and cross-reactivity in vivo. The three melanomas were highly immunogenic, and all induced some degree of protection against the other melanomas. Non-melanoma skin cancers induced by the same carcinogens were less immunogenic and did not immunize against the melanomas. We conclude that unlike other skin cancers, melanocytic tumors induced by chemical carcinogens and ultraviolet radiation are highly cross-reactive in vivo and thus represent a unique subset of murine skin cancers.
化学致癌物或紫外线辐射诱导小鼠产生的非黑色素瘤皮肤癌通常具有抗原性，但通过体内移植方法检测时，很少能诱导交叉保护性免疫。我们希望确定黑素细胞性皮肤肿瘤的表现是否类似，或者它们在体内是否表现出交叉反应性抗原。我们检测了通过紫外线辐射启动并用巴豆油促进、或用7,12-二甲基苯并[a]蒽启动并用12-O-十四酰佛波醇-13-乙酸酯或巴豆油加紫外线辐射促进，在C3H/HeNCr(MTV-)小鼠中诱导产生的三种黑色素瘤在体内的免疫原性和交叉反应性。这三种黑色素瘤具有高度免疫原性，并且都诱导了对其他黑色素瘤的一定程度的保护。由相同致癌物诱导产生的非黑色素瘤皮肤癌免疫原性较低，并且不能使小鼠对黑色素瘤产生免疫。我们得出结论，与其他皮肤癌不同，化学致癌物和紫外线辐射诱导产生的黑素细胞性肿瘤在体内具有高度交叉反应性，因此代表了小鼠皮肤癌的一个独特子集。
