Bernstein E F, Glass J M, DeGraff W G, Schlegel R, Black C, Fisher J M, Cook S N, Glatstein E, Russo A, Mitchell J B
Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md.
Arch Dermatol. 1991 May;127(5):683-7.
Photodynamic therapy involves the use of light of appropriate wavelength to excite a photosensitizer to result in tissue destruction. The photosensitizer dihematoporphyrin ether and red light were used to treat normal and human papilloma virus type 18-transfected keratinocytes in vitro. Although both cell lines were sensitive to treatment, normal keratinocytes retained more dihematoporphyrin ether and were more sensitive to photodynamic therapy than were transfected cells. In vitro data fail to show the selective retention of dihematoporphyrin ether reported elsewhere in the literature in papillomas and tumors. We did not find selective uptake or retention of dihematoporphyrin ether by human papilloma virus-transfected cells, despite previously published in vivo data to the contrary. Dihematoporphyrin ether retention and thus selective photosensitivity of papillomas in vivo is not caused by individual cellular differences in the processing of dihematoporphyrin ether. In addition, because in vitro results may not parallel in vivo results, both in vivo and in vitro models must be investigated in the study of phototherapeutic agents.
光动力疗法是利用适当波长的光激发光敏剂，从而导致组织破坏。使用光敏剂双血卟啉醚和红光在体外处理正常角质形成细胞和转染了18型人乳头瘤病毒的角质形成细胞。虽然两种细胞系对治疗均敏感，但正常角质形成细胞保留了更多的双血卟啉醚，并且比转染细胞对光动力疗法更敏感。体外数据未能显示文献中其他地方报道的双血卟啉醚在乳头瘤和肿瘤中的选择性保留。尽管先前发表的体内数据与此相反，但我们未发现转染了人乳头瘤病毒的细胞对双血卟啉醚有选择性摄取或保留。双血卟啉醚的保留以及因此乳头瘤在体内的选择性光敏性并非由双血卟啉醚处理过程中的个体细胞差异引起。此外，由于体外结果可能与体内结果不一致，因此在光疗药物研究中必须同时研究体内和体外模型。
