大鼠皮质大胶质细胞中免疫反应性GAP - 43表达的调节具有细胞类型特异性。
Regulation of immunoreactive GAP-43 expression in rat cortical macroglia is cell type specific.
作者信息
da Cunha A, Vitković L
机构信息
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Cell Biol. 1990 Jul;111(1):209-15. doi: 10.1083/jcb.111.1.209.
Growth-associated protein 43 (GAP-43) is an abundant, intensely investigated membrane phosphoprotein of the nervous system (Benowitz, L.I., and A. Routtenberg. 1987. Trends Neurosci. 10:527-532; Skene, J. H. P. 1989. Annu. Rev. Neurosci. 12:127-156), with a hitherto unknown function. We have previously demonstrated that astrocytes, brain macroglial cells, contain GAP-43 (Steisslinger, H. W., V. J. Aloyo, and L. Vitković, 1987. Brain Res. 415:375-379; Vitković, L., H. W. Steisslinger, V. J. Aloyo, and M. Mersel. 1988. Proc. Natl. Acad. Sci. USA. 85:8296-8300; Vitković L., and M. Mersel. 1989. Metab. Brain Dis. 4:47-53). Results from double immunofluorescent labeling experiments presented here show that oligodendrocytes also contain GAP-43 immunoreactivity (GAP-43ir). Thus, all three macroglial cell types of the central nervous system (type I and type 2 astrocytes and oligodendrocytes) contain GAP-43. Whereas immunoreactive GAP-43 is expressed by progenitors of all macroglial cell types, the developmental regulation of its expression is cell type specific. Immunoreactive GAP-43 is downregulated in type 1 astrocytes, and constitutively expressed in both type 2 astrocytes and oligodendrocytes. These results may be relevant to potential function(s) of GAP-43.
生长相关蛋白43（GAP - 43）是神经系统中一种含量丰富且受到广泛研究的膜磷蛋白（贝诺维茨，L.I.，和A.劳滕贝格。1987年。《神经科学趋势》10:527 - 532；斯凯内，J.H.P. 1989年。《神经科学年度评论》12:127 - 156），其功能至今尚不明确。我们之前已经证明，星形胶质细胞，即脑内的大胶质细胞，含有GAP - 43（施泰斯林格，H.W.，V.J.阿洛约，和L.维特科维奇，1987年。《脑研究》415:375 - 379；维特科维奇，L.，H.W.施泰斯林格，V.J.阿洛约，和M.默塞尔。1988年。《美国国家科学院院刊》85:8296 - 8300；维特科维奇，L.，和M.默塞尔。1989年。《脑代谢疾病》4:47 - 53）。此处展示的双重免疫荧光标记实验结果表明，少突胶质细胞也含有GAP - 43免疫反应性（GAP - 43ir）。因此，中枢神经系统的所有三种大胶质细胞类型（I型和II型星形胶质细胞以及少突胶质细胞）都含有GAP - 43。虽然所有大胶质细胞类型的祖细胞都表达免疫反应性GAP - 43，但其表达的发育调控具有细胞类型特异性。免疫反应性GAP - 43在I型星形胶质细胞中表达下调，而在II型星形胶质细胞和少突胶质细胞中持续表达。这些结果可能与GAP - 43的潜在功能相关。
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