He Jianghong, Liu Jun, Zhang Zhijun, Sun Maomin, Zhu Ting, Xia Chunlin
Cytoneurobiology Unit & Laboratory of Aging and Nervous Diseases, Medical College of Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, JS, 215123, People's Republic of China.
Mol Cell Biochem. 2009 May;325(1-2):159-67. doi: 10.1007/s11010-009-0030-8. Epub 2009 Feb 8.
Astrocytes play a more important role than simply providing physical support for neurons, however, the function(s) of type 1 and type 2 astrocytes (T1As, T2As), remains unclear. A DNA microarray was used to identify gene expression in cultured T1As and T2As isolated from postnatal day 1 rat cortex. Ninety-nine of the 138 differentially expressed genes were involved in a diverse number of processes. The fasciculation and elongation protein zeta-1 (FEZ1) gene was studied further because it has been suggested that it is not expressed by astrocytes. RT-PCR and Western blots confirmed the microarray data and showed that FEZ1 was present in T1 and T2As and is more highly expressed in T2As. Immunocytochemistry revealed that FEZ1 was located in the astrocytic cytoplasm and cell processes but not the nucleus. The results contribute to a clearer understanding of the two types of astrocytes.
然而，星形胶质细胞所起的作用不仅仅是为神经元提供物理支持，1型和2型星形胶质细胞（T1A、T2A）的功能仍不清楚。利用DNA微阵列来鉴定从出生后第1天大鼠皮质分离的培养T1A和T2A中的基因表达。138个差异表达基因中的99个参与了多种过程。对成束和延伸蛋白ζ-1（FEZ1）基因进行了进一步研究，因为有研究表明星形胶质细胞不表达该基因。逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹证实了微阵列数据，并表明FEZ1存在于T1A和T2A中，且在T2A中表达更高。免疫细胞化学显示FEZ1位于星形胶质细胞的细胞质和细胞突起中，而不在细胞核中。这些结果有助于更清楚地了解这两种类型的星形胶质细胞。
