Skidmore J A, Billah M
Camel Reproduction Centre, PO Box 79914, Dubai, United Arab Emirates.
Reprod Fertil Dev. 2011;23(3):438-43. doi: 10.1071/RD10136.
The aim of the present study was to investigate the use of exogenous progesterone and equine chorionic gonadotrophin (eCG) in non-ovulated and ovulated, asynchronous dromedary camel recipients being prepared for an embryo transfer programme. The uteri of 12 mated donor camels were flushed non-surgically 7 days after ovulation and 42 embryos were recovered. In Experiment 1, 16 embryos were transferred non-surgically to recipients on Day 3 or 4 after ovulation (ov+3 and ov+4, respectively). Each recipient received a daily dose of 75 mg, i.m., progesterone-in-oil from 2 days before embryo transfer until 6 days after ovulation. Thereafter, the progesterone dose was reduced to 50 mg on Day 7 and finally to 25 mg day(-1) on Days 8 and 9. Nine of 16 recipients (56%; ov+3, n=4; ov+4, n=5) became pregnant compared with none of eight non-progesterone treated controls, into which embryos were transferred on Day 4 after ovulation. In Experiment 2, 18 non-ovulated recipients received 75 mg, i.m., progesterone-in-oil daily from 3 days before until 12 days after non-surgical transfer of a Day 7 blastocyst, at which time pregnancy was diagnosed by ultrasonography. All pregnant recipients continued to receive 75 mg progesterone-in-oil daily for a further 6 days, when each camel received 2000 IU, i.m., eCG. Progesterone treatment was then reduced to 50 mg day(-1) and, when a follicle(s) ≥1.3 cm in diameter were present in the ovaries, each animal received 20 μg buserelin to induce ovulation. Once the corpora lutea had developed, progesterone treatment was reduced to 25 mg day(-1) for a final 3 days. Fourteen of 18 recipients (78%) became pregnant and seven of these (50%) remained pregnant after eCG treatment. Of the seven pregnancies that were lost, two were lost before eCG treatment, two did not respond to eCG treatment and three responded to eCG treatment and ovulated, but lost their pregnancies 6-8 days after the last progesterone injection.
本研究的目的是调查在为胚胎移植计划做准备的未排卵和已排卵、不同步的单峰驼受体中使用外源性孕酮和马绒毛膜促性腺激素（eCG）的情况。12只已交配供体骆驼在排卵后7天进行非手术子宫冲洗，回收了42枚胚胎。在实验1中，16枚胚胎在排卵后第3天或第4天（分别为ov+3和ov+4）非手术移植到受体中。从胚胎移植前2天到排卵后6天，每个受体每天肌肉注射75毫克油剂孕酮。此后，孕酮剂量在第7天降至50毫克，最后在第8天和第9天降至25毫克/天。16只受体中有9只（56%；ov+3组，n = 4；ov+4组，n = 5）怀孕，而8只未接受孕酮治疗的对照组（在排卵后第4天移植胚胎）无一怀孕。在实验2中，18只未排卵受体从非手术移植第7天囊胚前3天到移植后12天，每天肌肉注射75毫克油剂孕酮，此时通过超声检查诊断怀孕。所有怀孕受体继续每天肌肉注射75毫克油剂孕酮6天，之后每只骆驼肌肉注射2000国际单位eCG。然后孕酮治疗降至50毫克/天，当卵巢中出现直径≥1.3厘米的卵泡时，每只动物注射20微克布舍瑞林诱导排卵。一旦黄体形成，孕酮治疗在最后3天降至25毫克/天。18只受体中有14只（78%）怀孕，其中7只（50%）在eCG治疗后仍保持怀孕。在失去的7次妊娠中，2次在eCG治疗前失去，2次对eCG治疗无反应，3次对eCG治疗有反应并排卵，但在最后一次注射孕酮后6 - 8天失去妊娠。
