[基于银纳米颗粒的表面增强拉曼光谱法快速测定宠物食品中的三聚氰胺]
[Rapid determination of melamine in pet food by surface enhanced Raman spectroscopy in combination with Ag nanoparticles].
作者信息
Cheng Jie, Su Xiao-Ou
机构信息
Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, National Feed Quality Control Center, Beijing 100081, China.
出版信息
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 Jan;31(1):131-5.
The rapid qualitative and quantitative analysis of melamine in pet food was realized by surface-enhanced Raman spectroscopy in combination with Ag nanoparticle. In the present study, the 709 and 1 542 cm(-1) Raman shift was chosen as qualitative basis. The quantitative calculation of the concentration range between 1.0 and 10.0 mg x kg(-1) was achieved based on the intensity of 1 149 cm(-1) Raman peak which was used as a normalization standard. The limit of detection was 0.5 mg x kg(-1). The Ag nanoparticle had a strong Raman enhancement effect on melamine and the intensity was affected by the adding time of Ag nanoparticle and the vortex strength. At the same time, the intensity of SERS was affected by the extraction solvent type, and the manner of extraction. The analysis time of each sample was about 5 min. It was so quick that it was easy to realize the rapid detection of melamine in pet food compared with existing methods.
通过表面增强拉曼光谱法结合银纳米颗粒实现了宠物食品中三聚氰胺的快速定性和定量分析。在本研究中，选择709和1542cm⁻¹的拉曼位移作为定性依据。基于用作归一化标准的1149cm⁻¹拉曼峰的强度，实现了1.0至10.0mg·kg⁻¹浓度范围内的定量计算。检测限为0.5mg·kg⁻¹。银纳米颗粒对三聚氰胺具有很强的拉曼增强效应，其强度受银纳米颗粒添加时间和涡旋强度的影响。同时，表面增强拉曼光谱的强度受萃取溶剂类型和萃取方式的影响。每个样品的分析时间约为5分钟。与现有方法相比，该方法速度非常快，易于实现宠物食品中三聚氰胺的快速检测。
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