School of Life Sciences, East China Normal University, Shanghai, China.
Mol Ecol Resour. 2011 Jan;11(1):206-10. doi: 10.1111/j.1755-0998.2010.02906.x. Epub 2010 Aug 29.
Studies on feeding habits based on faeces dissecting are imperative to understand the natural history of Tibetan foxes and their functions in the transmission of a lethal zoonotic parasite, Echinococcus multilocularis. However, Tibetan foxes and red foxes live sympatrically on the Tibetan plateau, China. Therefore, the faeces of Tibetan foxes must be distinguished from those of red foxes. We established a diagnostic method to distinguish the faeces of the two species by amplifying a portion of the mitochondrial cytochrome b gene (cytb) and digesting with the restriction enzymes BamHI and SspI, to produce specific diagnostic banding patterns. This PCR-RFLP assay enabled rapid, accurate and easily performed identification and differentiation of the two species.
基于粪便解剖的食性研究对于了解西藏狐的自然历史及其在致命的人畜共患寄生虫——多房棘球绦虫传播中的作用至关重要。然而，西藏狐和赤狐在中国青藏高原上共生。因此，必须区分西藏狐和赤狐的粪便。我们建立了一种通过扩增线粒体细胞色素 b 基因（cytb）的一部分并使用 BamHI 和 SspI 限制酶进行消化来区分两种物种粪便的诊断方法，从而产生特定的诊断带型。这种 PCR-RFLP 检测方法可快速、准确且易于进行两种物种的鉴定和区分。
