Qingqiu Zuo, Xiaohui Sun, Xu Wang, Xiaodong Weng, Xiaoming Wang, Youzhong Ding, Fei Xie, Jianfeng Ba, Bin Zou, Wenming Tan, Zhenghuan Wang
School of Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai, 200241, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, Key Laboratory of Parasite and Vector Biology, Ministry of Health, 207 Ruijin Rd, Shanghai, 200025, China.
Int J Parasitol Parasites Wildl. 2020 Jul 8;12:242-249. doi: 10.1016/j.ijppaw.2020.06.008. eCollection 2020 Aug.
Tibetan foxes () have been confirmed as the main wild definitive hosts in echinococcosis transmission in the eastern Tibetan Plateau. However, little information is available about the epidemiology in wildlife from the perspective of the Taeniidae family, which is essential knowledge in understanding the epidemiology and phylogeography of cestode species in the Tibetan plateau. Therefore, in this study, we used copro-PCR techniques, by amplifying and gene fragments, to detect the taeniid species from Tibetan fox feces collected in Shiqu County, (Sichuan Province, China), eastern Tibetan Plateau. Phylogenetic relationships between amplified sequences and existed species genotypes were evaluated. Then, the maximum prevalence (positive PCR results from at least one primer pair) and the conservative prevalence (positive PCR results from at least two primer pairs) were calculated. Thirty-six Tibetan fox feces were analyzed. (conservative prevalence ± 95% CI: 22.2% ± 13.6%; maximum prevalence ± 95% CI: 33.3% ± 15.4%) and (2.8 ± 5.4%; 8.3 ± 9.0%) was detected. Meanwhile, DNA fragments of were detected with high similarity to NCBI sequences (, 94.0%) and to the larva sample DNA sequenced in this study (93.4%), and were supported by phylogenetic analysis. Thus, might infect Tibetan foxes (5.6% ± 7.5%, 11.1% ± 10.3%). Our limited findings in the epidemiology of parasitic species suggest that sylvatic transmission cycles for a more species-rich Taeniid community must be established between wild canids and small mammals than just for the two species. Besides, discrepancies in different primer pairs in detecting the taeniid species were evaluated. The sensitivity of some widely used universal primer pairs was poor in detecting species from canid copro-DNA samples. It is still challenging to the development of effective taeniid species-specific molecular markers especially for non-zoonotic species.
藏狐已被确认为青藏高原东部棘球蚴病传播的主要野生终末宿主。然而，从带科的角度来看，关于野生动物流行病学的信息很少，而这是了解青藏高原绦虫物种流行病学和系统地理学的重要知识。因此，在本研究中，我们使用粪便聚合酶链反应（copro-PCR）技术，通过扩增和基因片段，从青藏高原东部四川省石渠县收集的藏狐粪便中检测带科物种。评估扩增序列与现有物种基因型之间的系统发育关系。然后，计算最大流行率（至少一对引物对的阳性PCR结果）和保守流行率（至少两对引物对的阳性PCR结果）。对36份藏狐粪便进行了分析。检测到（保守流行率±95%置信区间：22.2%±13.6%；最大流行率±95%置信区间：33.3%±15.4%）和（2.8±5.4%；8.3±9.0%）。同时，检测到的DNA片段与NCBI序列（，94.0%）和本研究中测序的幼虫样本DNA（93.4%）具有高度相似性，并得到系统发育分析的支持。因此，可能感染藏狐（5.6%±7.5%，11.1%±10.3%）。我们在寄生带科物种流行病学方面的有限发现表明，与仅涉及两种物种相比，必须在野生犬科动物和小型哺乳动物之间建立一个物种更丰富的带科群落的野生动物传播循环。此外，评估了不同引物对在检测带科物种方面的差异。一些广泛使用的通用引物对在检测犬科动物粪便DNA样本中的带科物种时灵敏度较差。开发有效的带科物种特异性分子标记仍然具有挑战性，尤其是对于非人畜共患物种。
