一种用于多种禽类物种性别鉴定的非侵入性、直接实时 PCR 方法。
A noninvasive, direct real-time PCR method for sex determination in multiple avian species.
机构信息
U.S. Geological Survey Patuxent Wildlife Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
出版信息
Mol Ecol Resour. 2011 Mar;11(2):415-7. doi: 10.1111/j.1755-0998.2010.02951.x. Epub 2010 Dec 12.
Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
基于聚合酶链式反应（PCR）的鸟类性别鉴定方法已经成熟，自 20 世纪 90 年代首次引入以来，几乎没有什么变化。虽然这些方法允许对以前难以分析的物种进行性别鉴定，但由于 DNA 提取和凝胶电泳繁琐，它们不适合高通量分析。我们开发了一种基于高通量实时 PCR 的鸟类性别分析方法，该方法采用非侵入性样本采集，避免了 DNA 提取和凝胶电泳。
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