Majchrakova Zuzana, Bielikova Marcela, Hrckova Turnova Evelina, Gasparkova Petra, Turna Jan, Dudas Andrej
Slovgen Ltd., Ilkovicova 8, 841 04 Bratislava, Slovakia.
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, 842 15 Bratislava, Slovakia.
Animals (Basel). 2024 Jun 7;14(12):1719. doi: 10.3390/ani14121719.
Sex determination based just on morphological traits such as plumage dichromatism, sexual size dimorphism, behavior, or vocalizations is really challenging because of the sexual monomorphism present in more than half of avian species. Currently, a lot of them can be tested through DNA-based procedures, but they do not fit all the avian species, such as . The aim of this study was to design a new molecular method suitable for routine sex determination for that species that is fast, simple, and cost- and time- effective. DNA was isolated from dry blood stain and/or chest feather samples of species. We used two sets of sex-specific primers (ZF/ZR and WF/WR) to amplify the expected fragments localized on the highly conserved gene to distinguish between sexes due to the W-specific DNA sequence present only in females. We confirmed the accuracy and consistency of the PCR-based method based on length differences to distinguish between the sexes of , which amplified two fragments in females and one fragment in males.
仅基于诸如羽毛二态性、两性体型差异、行为或发声等形态特征来确定性别极具挑战性，因为超过半数的鸟类物种存在性别单态性。目前，很多物种可以通过基于DNA的方法进行检测，但这些方法并不适用于所有鸟类物种，例如 。本研究的目的是设计一种新的分子方法，适用于该物种的常规性别鉴定，该方法快速、简单，且具有成本效益和时间效益。从 物种的干血斑和/或胸部羽毛样本中提取DNA。我们使用两组性别特异性引物（ZF/ZR和WF/WR）来扩增位于高度保守的 基因上的预期片段，以区分性别，因为W特异性DNA序列仅存在于雌性中。我们基于长度差异证实了基于PCR的方法在区分 物种性别的准确性和一致性，该方法在雌性中扩增出两个片段，在雄性中扩增出一个片段。
