Finch J L, Hope R M, van Daal A
Genetics Department, University of Adelaide, SA, Australia.
Sci Justice. 1996 Apr-Jun;36(2):93-5. doi: 10.1016/S1355-0306(96)72572-8.
A sex determination method has been developed using the polymerase chain reaction (PCR) which involved the amplification of the sex-determining region Y (SRY) gene and the amplification of the HLA-DQa gene as an internal control. This method, which can be applied to old and degraded DNA samples, allowed confident sexing of biological samples producing readily identifiable PCR products using two sets of primers in the one PCR reaction. The PCR products were short DNA sequences of 139bp and 239/242bp for the SRY and HLA-DQa regions respectively. The application of this method in forensic science will allow determination of the gender of perpetrators of crimes involving biological materials.
已开发出一种利用聚合酶链反应（PCR）的性别鉴定方法，该方法涉及对性别决定区Y（SRY）基因的扩增以及对HLA - DQa基因的扩增作为内部对照。这种方法可应用于陈旧和降解的DNA样本，通过在一个PCR反应中使用两组引物，能够对生物样本进行可靠的性别鉴定，产生易于识别的PCR产物。SRY和HLA - DQa区域的PCR产物分别是139bp和239/242bp的短DNA序列。该方法在法医学中的应用将有助于确定涉及生物材料的犯罪行为人的性别。
