The association of HLA class II DR B1 alleles with HCV infection in Egyptian children.

BACKGROUND AND STUDY AIMS

Human leucocyte antigens (HLA) class II appear to play an important role in the individual's immune response to viral infection. The aim of this study is to assess the relationship between HLA class II antigens with the clinical, laboratory and histopathological state of the liver in Egyptian children and adolescents with chronic hepatitis C virus (HCV) infection.

PATIENTS AND METHODS

The study included 46 chronically infected HCV children and adolescents without - hepatitis B virus (HBV) nor human immunodeficiency virus - (HIV). Their mean age was 10.4±4.23years (3-17). HLA-DRB typing was done by polymerase chain reaction (PCR) for the patients and 20 control subjects. Biochemical and haematological parameters were assessed as well as a liver biopsy was taken from the included patients.

RESULTS

The most frequent alleles demonstrated among patients were DRB1∗03, DRB1∗04 and DRB1∗13 (45.6%, 39.1% and 26.1%), respectively. Analysis of DRB1 frequencies between patients and control revealed that DRB115 is significantly reduced among patients when compared with the control group (p<0.01). Patients possessing the allele DRB103 had significantly reduced platelet count (p=0.03), and this allele was presented to a greater extent in patients with minimal grade of inflammation. Patients with DRB104 had significantly low serum albumin (p=0.04) and patients with DRB113 had significantly high serum aspartate aminotransferase (AST) levels (p=0.05).

CONCLUSION

In Egyptian HCV-infected children, special HLA patterns were found; HLA DRB103 was present in nearly half of the patients, while the frequency of HLA DRB115 was significantly reduced among the cases in comparison to the control subjects.