Clinical Immunology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Scand J Immunol. 2010 Dec;72(6):548-53. doi: 10.1111/j.1365-3083.2010.02468.x.
Egypt has the highest prevalence of hepatitis C virus (HCV) in the world, ranging from 6% to 28% with an average of approximately 13.8% in the general population. It has been reported that human leucocyte antigen (HLA) alleles are associated with the outcome of HCV infection, but this associations showed ethnic and geographical differences. The objective of this study is to investigate the association between the frequencies of HLA Class I and chronic HCV infection in Egyptian patients and to find out whether there is a relation between certain HLA Class I antigens and HCV viral load, degree of fibrosis, activity and alanine aminotransferase (ALT) level. A case control study was conducted on 100 patients with chronic HCV infection and 150 healthy controls. HLA-A and HLA-B typing by complement-dependent micro-lympho-cytotoxicity assay was performed for both groups. HLA-A11 antigen was significantly increased in patients with chronic HCV infection versus controls (OR 3.98; 95% CI = 1.85-8.89; P = 0.001; and Pc = 0.021). HLA-B12, HLA-B13, HLA-B17 and HLA-B40 were higher in patients, and HLA-A32 and HLA-B14 were higher in controls, although the significance was lost after correction for multiple testing. HLA-A9 was significantly associated with low viral load (P = 0.008, Pc = 0.048). The results of this work implicate that HLA-A11 antigen may influence chronic HCV infection and may play a role in viral persistence. Different HLA Class I antigens are not associated with degree of liver fibrosis, grades of activity or level of ALT. However, HLA-A9 is associated with low HCV viral load in chronic HCV Egyptian patients.
埃及是世界上丙型肝炎病毒 (HCV) 流行率最高的国家，普通人群中的 HCV 流行率为 6%至 28%，平均约为 13.8%。据报道，人类白细胞抗原 (HLA) 等位基因与 HCV 感染的结果有关，但这种关联存在种族和地域差异。本研究的目的是探讨埃及丙型肝炎患者 HLA Ⅰ类频率与慢性 HCV 感染之间的关系，并确定某些 HLA Ⅰ类抗原与 HCV 病毒载量、纤维化程度、活动度和丙氨酸氨基转移酶 (ALT) 水平之间是否存在关系。对 100 例慢性 HCV 感染患者和 150 例健康对照进行了病例对照研究。对两组进行了 HLA-A 和 HLA-B 补体依赖性微量淋巴细胞毒性试验。与对照组相比，慢性 HCV 感染患者的 HLA-A11 抗原明显增加（OR 3.98；95%CI = 1.85-8.89；P = 0.001；Pc = 0.021）。HLA-B12、HLA-B13、HLA-B17 和 HLA-B40 在患者中较高，HLA-A32 和 HLA-B14 在对照组中较高，但经多次检验校正后意义丧失。HLA-A9 与低病毒载量显著相关（P = 0.008，Pc = 0.048）。本研究结果表明，HLA-A11 抗原可能影响慢性 HCV 感染，并可能在病毒持续存在中发挥作用。不同的 HLA Ⅰ类抗原与肝纤维化程度、活动度或 ALT 水平无关。然而，HLA-A9 与埃及慢性 HCV 患者的低 HCV 病毒载量有关。
