Wegener G, Krause U, Thuy M
Institut für Zoologie, Johannes Gutenberg Universität, Mainz, FRG.
FEBS Lett. 1990 Jul 16;267(2):257-60. doi: 10.1016/0014-5793(90)80939-g.
Glycolytic flux in skeletal muscle is controlled by 6-phosphofructokinase but how this is achieved is controversial. Brief exercise (swimming) in frogs caused a dramatic increase in the phosphofructokinase activator, fructose 2,6-bisphosphate, in working muscle. The kinetics of phosphofructokinase suggest that in resting muscle, the enzyme is inhibited by ATP plus citrate and that the increase in fructose 2,6-bisphosphate is part of the mechanism to activate phosphofructokinase when exercise begins. When exercise was sustained, fructose 2,6-bisphosphate in muscle was decreased as was the rate of lactate accumulation. Glycolytic flux and the content of fructose 2,6-bisphosphate appear to be closely correlated in working frog muscle in vivo.
骨骼肌中的糖酵解通量受6-磷酸果糖激酶控制，但具体实现方式存在争议。青蛙的短暂运动（游泳）会使运动肌肉中的磷酸果糖激酶激活剂果糖2,6-二磷酸急剧增加。磷酸果糖激酶的动力学表明，在静息肌肉中，该酶受到ATP和柠檬酸的抑制，而果糖2,6-二磷酸的增加是运动开始时激活磷酸果糖激酶机制的一部分。当运动持续时，肌肉中的果糖2,6-二磷酸减少，乳酸积累速率也降低。在体内工作的青蛙肌肉中，糖酵解通量与果糖2,6-二磷酸的含量似乎密切相关。
