正常血液中CD4+CD8-、CD4-CD8+、CD4-CD8dim+和CD4-CD8-淋巴细胞的膜TcRαβ和TcRγδ链表达模式

Patterns of membrane TcR alpha beta and TcR gamma delta chain expression by normal blood CD4+CD8-, CD4-CD8+, CD4-CD8dim+ and CD4-CD8- lymphocytes.

作者信息

Scott C S, Richards S J, Roberts B E

机构信息

Department of Haematology, Cookridge Hospital, Leeds, U.K.

出版信息

Immunology. 1990 Jul;70(3):351-6.

PMID:2143170

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1384165/

Abstract

Enriched CD4+CD8-/CD4-CD8-, CD4-CD8+/CD4-CD8- and CD4-CD8- cell suspensions were prepared from normal peripheral blood by selective immunomagnetic depletion of monoclonal antibody-defined lymphocyte populations. Subsequent examination of these modified cell fractions by two-colour flow cytometry provided a means of determining the expression of membrane T-cell receptor (TcR)alpha beta and TcR gamma delta chains by both major (CD4+ and CD8+) and minor (CD3+CD4-CD8dim+ and CD3+CD4-CD8-) lymphocyte subpopulations. Normal CD4+CD8- lymphocytes were almost invariably (greater than 99%) TcR alpha beta+, whereas lymphocytes expressing membrane CD8, which could be further subdivided according to differences in fluorescent staining intensity into CD3+CD4-CD8+, CD3+CD4-CD8dim+ and CD3-CD4-CD8dim+ components, were characterized by distinct differences in patterns of TcR chain expression. In contrast to CD3+CD4-CD8+ cells, which were predominantly (99%) TcR alpha beta+, CD3+CD4-CD8dim+ lymphocytes showed a significant proportion (33%) of TcR gamma delta+ cells (natural killer-associated CD3-CD4-CD8dim+ cells were uniformly TcR-). The highest proportion (62%) of TcR gamma delta+ cells was associated with the CD3+CD4-CD8- fraction, but these studies also revealed that a significant minority of this population was TcR alpha beta+. Despite some evidence for normal inter-individual variation, further analysis of membrane CD8 fluorescent intensities confirmed clear differential relationships for TcR alpha beta and TcR gamma delta chain expression.

摘要

通过对单克隆抗体定义的淋巴细胞群体进行选择性免疫磁珠去除，从正常外周血中制备了富集的CD4⁺CD8⁻/CD4⁻CD8⁻、CD4⁻CD8⁺/CD4⁻CD8⁻和CD4⁻CD8⁻细胞悬液。随后通过双色流式细胞术对这些经过修饰的细胞组分进行检测，提供了一种确定主要（CD4⁺和CD8⁺）和次要（CD3⁺CD4⁻CD8dim⁺和CD3⁺CD4⁻CD8⁻）淋巴细胞亚群中膜T细胞受体（TcR）αβ和TcRγδ链表达的方法。正常CD4⁺CD8⁻淋巴细胞几乎总是（大于99%）TcRαβ⁺，而表达膜CD8的淋巴细胞，根据荧光染色强度的差异可进一步细分为CD3⁺CD4⁻CD8⁺、CD3⁺CD4⁻CD8dim⁺和CD3⁻CD4⁻CD8dim⁺组分，其特征在于TcR链表达模式存在明显差异。与主要为（99%）TcRαβ⁺的CD3⁺CD4⁻CD8⁺细胞不同，CD3⁺CD4⁻CD8dim⁺淋巴细胞显示出相当比例（33%）的TcRγδ⁺细胞（自然杀伤相关的CD3⁻CD4⁻CD8dim⁺细胞均为TcR⁻）。TcRγδ⁺细胞比例最高（62%）与CD3⁺CD4⁻CD8⁻组分相关，但这些研究也表明该群体中有相当一部分少数细胞为TcRαβ⁺。尽管有证据表明个体间存在正常变异，但对膜CD8荧光强度的进一步分析证实了TcRαβ和TcRγδ链表达之间存在明显的差异关系。