IBB-Institute for Biotechnology and Bioengineering, Center of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal.
Biotechnol Lett. 2011 Aug;33(8):1601-6. doi: 10.1007/s10529-011-0593-9. Epub 2011 Mar 24.
Oil-in-water emulsions composed of methyl ricinoleate (MR) or castor oil (CO) as the organic phase, stabilized by Tween 80, are in the basis of the biotechnological production of γ-decalactone. Yarrowia lipolytica was used due to its ability to grow on hydrophobic substrates and to carry out the biotransformation. The characterization of oil droplets size distribution by laser granulometry was performed under different oil concentrations. The impact of the presence of cells on droplets size was also analyzed as well as the relevance of washing inoculum cells. Furthermore, the granulometric characterization of the emulsions was related with γ-decalactone production and it was observed that, in the presence of non-washed cells, the smaller droplets disappeared, using both oils, which increased γ-decalactone concentration. This suggests that the access of cells to the substrate occurs by their adhesion around larger oil droplets.
由甲基蓖麻醇酸酯 (MR) 或蓖麻油 (CO) 组成的油包水乳状液作为有机相，由吐温 80 稳定，是生物技术生产γ-癸内酯的基础。由于其能够在疏水性基质上生长并进行生物转化，因此使用了假丝酵母。通过激光粒度法对不同油浓度下的油滴大小分布进行了表征。还分析了细胞存在对液滴大小的影响以及洗涤接种细胞的相关性。此外，乳化液的粒度表征与γ-癸内酯的生产有关，并且观察到，在存在未洗涤细胞的情况下，两种油都使用较小的液滴消失，从而增加了γ-癸内酯的浓度。这表明细胞通过围绕较大的油滴附着来接触底物。
