Pereira de Andrade Dayana, Carvalho Beatriz Ferreira, Schwan Rosane Freitas, Dias Disney Ribeiro
Department of Biology, Federal University of Lavras, Campus Universitario, BR-37200-000, Lavras, MG, Brazil.
Department of Animal Science, Federal University of Lavras, Campus Universitario, BR-37200-000, Lavras, MG, Brazil.
Food Technol Biotechnol. 2017 Jun;55(2):225-230. doi: 10.17113/ftb.55.02.17.5009.
γ-Decalactone is a flavour compound that when obtained by biotechnological production using microorganisms is classified as natural. The aim of this study is to evaluate various conditions for γ-decalactone production by tropical yeast strains CCMA 0242 and CCMA 0243. The growth of and γ-decalactone production by CCMA 0242 were higher in castor oil than in glycerol. γ-Decalactone production in single batch or fed-batch fermentation did not differ significantly. The γ-decalactone production by CCMA 0243 was better at initial pH=5, while the production by CCMA 0242 was better at initial pH=6. The yeast CCMA 0243 produced more γ-decalactone than CCMA 0242 under the same fermentation conditions. The crude glycerol was not an alternative substrate for γ-decalactone production by CCMA 0242. Castor oil at volume fraction of 30% showed better results as a substrate. The strain CCMA 0243 showed better results of γ-decalactone production. This yeast species can be considered an alternative producer of γ-decalactone in biotechnological processes.
γ-癸内酯是一种风味化合物，当通过微生物生物技术生产获得时，它被归类为天然产物。本研究的目的是评估热带酵母菌株CCMA 0242和CCMA 0243生产γ-癸内酯的各种条件。CCMA 0242在蓖麻油中的生长和γ-癸内酯产量高于在甘油中的。单批次或补料分批发酵中γ-癸内酯的产量没有显著差异。CCMA 0243在初始pH = 5时γ-癸内酯产量较好，而CCMA 0242在初始pH = 6时产量较好。在相同发酵条件下，酵母CCMA 0243产生的γ-癸内酯比CCMA 0242更多。粗甘油不是CCMA 0242生产γ-癸内酯的替代底物。体积分数为30%的蓖麻油作为底物显示出更好的结果。菌株CCMA 0243在γ-癸内酯生产方面显示出更好的结果。这种酵母物种可被视为生物技术过程中γ-癸内酯的替代生产者。
