School of Environmental Sciences, University of Guelph, ON, Canada.
Antonie Van Leeuwenhoek. 2011 Aug;100(2):183-95. doi: 10.1007/s10482-011-9577-y. Epub 2011 Mar 24.
The effect of glucose addition (0 and 500 μg C g(-1) soil) and nitrate (NO(3)) addition (0, 10, 50 and 500 μg NO(3)-N g(-1) soil) on nitric oxide reductase (cnorB) gene abundance and mRNA levels, and cumulative denitrification were quantified over 48 h in anoxic soils inoculated with Pseudomonas mandelii. Addition of glucose-C significantly increased cnorB(p) (P. mandelii and related species) mRNA levels and abundance compared with soil with no glucose added, averaged over time and NO(3) addition treatments. Without glucose addition, cnorB(p) mRNA levels were higher when 500 μg NO(3)-N g(-1) soil was added compared with other NO(3) additions. In treatments with glucose added, addition of 50 μg NO(3)-N g(-1) soil resulted in higher cnorB(p) mRNA levels than soil without NO(3) but was not different from the 10 and 500 μg NO(3)-N g(-1) treatments. cnorB(p) abundance in soils without glucose addition was significantly higher in soils with 500 μg NO(3)-N g(-1) soil compared to lower N-treated soils. Conversely, addition of 500 μg NO(3)-N g(-1) soil resulted in lower cnorB(p) abundance compared with soil without N-addition. Over 48 h, cumulative denitrification in soils with 500 μg glucose-C g(-1) soil, and 50 or 500 μg NO(3)-N g(-1) was higher than all other treatments. There was a positive correlation between cnorB(p) abundance and cumulative denitrification, but only in soils without glucose addition. Glucose-treated soils generally had higher cnorB(p) abundance and mRNA levels than soils without glucose added, however response of cnorB(p) abundance and mRNA levels to NO(3) supply depended on carbon availability.
在添加葡萄糖（0 和 500μg C g（-1）土壤）和硝酸盐（NO（3））（0、10、50 和 500μg NO（3）-N g（-1）土壤）的情况下，葡萄糖-C 的添加显着增加了 cnorB（p）（假单胞菌）mRNA 水平和丰度与没有添加葡萄糖的土壤相比，随着时间和 NO（3）添加处理的平均值。在没有添加葡萄糖的情况下，当添加 500μg NO（3）-N g（-1）土壤时，cnorB（p）mRNA 水平高于其他 NO（3）添加物。在添加葡萄糖的处理中，添加 50μg NO（3）-N g（-1）土壤导致 cnorB（p）mRNA 水平高于没有 NO（3）的土壤，但与 10 和 500μg NO（3）-N g（-1）处理无差异。在没有葡萄糖添加的土壤中，与低氮处理的土壤相比，添加 500μg NO（3）-N g（-1）土壤导致 cnorB（p）丰度显着增加。相反，与不添加氮的土壤相比，添加 500μg NO（3）-N g（-1）土壤导致 cnorB（p）丰度降低。在添加 500μg 葡萄糖-C g（-1）土壤和 50 或 500μg NO（3）-N g（-1）的土壤中，48 小时内的累积脱氮量高于所有其他处理。cnorB（p）丰度与累积脱氮量之间存在正相关关系，但仅在没有葡萄糖添加的土壤中。与没有添加葡萄糖的土壤相比，添加葡萄糖的土壤通常具有更高的 cnorB（p）丰度和 mRNA 水平，但是 cnorB（p）丰度和 mRNA 水平对 NO（3）供应的反应取决于碳的可用性。
