Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang, 471023, China,
World J Microbiol Biotechnol. 2014 Feb;30(2):389-97. doi: 10.1007/s11274-013-1457-3. Epub 2013 Aug 11.
To understand the effects of planting tree peony (Paeonia suffruticosa) on soil microbial community structure, soil samples were collected from the tree peony gardens with three peony cultivars and three planting years, and adjacent wasteland at Luoyang, Henan Province of China. Soil microbial communities were analyzed by the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of partial 16S rDNA and sequencing methods. With the succeeding development of tree peony garden ecosystems, soil pH, organic C, total P, and available P increased. Soil total N, the cell numbers of bacteria, fungi, and actinomycetes, the Shannon's diversity index (H), richness (S), and Evenness (E(H)) first showed an increasing trend after wasteland was reclaimed and then a decreasing trend became apparent after 5 years of planting. Principal component analysis based on DGGE banding patterns showed that the microbial community structures were influenced by tree peony cultivars and planting years, and the influences of planting years were greater than those of tree peony cultivars. Sequence analysis of the DGGE bands revealed that the dominant bacteria in tree peony garden soils belonged to Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Planctomycetes, Euryarchaeota, and Unclassified-bacteria. Moreover, some bacteria closely related to Bradyrhizobium, Sphingopyxis, Novosphingobium, and Sphingomonas, which have been associated with nitrogen fixation and recalcitrant compounds degradation, disappeared with the increasing planting years of tree peony. The bacteria had similarity of 100% compared with Pseudomonas mandelii which was a denitrifying bacteria, and increased gradually with increasing planting years of tree peony.
为了了解牡丹（Paeonia suffruticosa）种植对土壤微生物群落结构的影响，从河南省洛阳市的牡丹园采集了三个牡丹品种和三个种植年份的土壤样本，并采集了相邻的荒地。通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）和测序方法分析土壤微生物群落。随着牡丹园生态系统的不断发展，土壤 pH 值、有机碳、总磷和有效磷增加。土壤总氮、细菌、真菌和放线菌的细胞数、香农多样性指数（H）、丰富度（S）和均匀度（E（H））在开垦荒地后先呈现增加趋势，然后在种植 5 年后又呈现减少趋势。基于 DGGE 条带模式的主成分分析表明，微生物群落结构受牡丹品种和种植年限的影响，种植年限的影响大于牡丹品种的影响。DGGE 条带的序列分析表明，牡丹园土壤中的优势细菌属于变形菌门、拟杆菌门、放线菌门、厚壁菌门、酸杆菌门、浮霉菌门、广古菌门和未分类细菌门。此外，一些与固氮和难降解化合物降解有关的细菌，如Bradyrhizobium、Sphingopyxis、Novosphingobium 和 Sphingomonas，随着牡丹种植年限的增加而消失。与脱氮菌Pseudomonas mandelii 的相似度为 100%，且随着牡丹种植年限的增加而逐渐增加。
