宽叶豆甲醇提取物对细胞生长和抗氧化酶活性的影响。

Effects of methanolic extracts from broad beans on cellular growth and antioxidant enzyme activity.

机构信息

Nutrition Section, Yms Laboratory, Gifu, Japan,

出版信息

Environ Health Prev Med. 2007 Nov;12(6):251-7. doi: 10.1007/BF02898032.

DOI:10.1007/BF02898032

PMID:21432071

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2723485/

Abstract

OBJECTIVE

There are several reports of cellular-aging-dependent alterations in the antioxidant capacity of human fibroblasts. Fibroblasts show slower the growth rate at late passages (referred to hereafter as old cells) than at early passages (referred to hereafter as young cells). Antioxidants may control cellular growth by modulating reactive oxygen species (ROS). Methanolic extracts from broad beans (MEBB) contain phenolic compounds and have ROS-scavenging activities. In this study, we investigated the effects of MEBB on cellular growth and antioxidant levels in normal human lung fibroblasts.

METHODS

To determine cytosolic superoxide dismutase (SOD) activities, cytosolic glutathione peroxidase (GSH-Px) activities, catalase activities, reduced glutathione (GSH) concentrations, and growth rate, MEBB treatments were performed on young and old cells.

RESULTS

In young and old cells treated with 120 μg/ml MEBB, the growth rates increased by 28.1 and 15.2%, respectively, compared with controls. The MEBB treatment of young cells caused a 62.5% increase in SOD activity, but the treatment of old cells caused a 39.5% decrease. The catalase activities of the young and old cells treated with MEBB were equal to those of control cells. Young and old cells treated with MEBB were equal to the control cells in terms of GSH-Px activity. The GSH concentrations in the young and old cells treated with 120 μg/ml MEBB increased by 22.1 and 45.9%, respectively.

CONCLUSION

These studies elucidated a new cellular growth mechanism whereby human lung fibroblasts modulate intracellular GSH levels via the action of MEBB.

摘要

目的

有几项报告表明，人类成纤维细胞的抗氧化能力会随着细胞衰老而发生改变。成纤维细胞在晚期传代（以下称为老年细胞）时的生长速度比早期传代（以下称为年轻细胞）慢。抗氧化剂可以通过调节活性氧（ROS）来控制细胞生长。蚕豆的甲醇提取物（MEBB）含有酚类化合物，具有清除 ROS 的活性。在这项研究中，我们研究了 MEBB 对正常人类肺成纤维细胞的细胞生长和抗氧化水平的影响。

方法

为了测定胞浆中超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GSH-Px）活性、过氧化氢酶活性、还原型谷胱甘肽（GSH）浓度和生长速度，对年轻和老年细胞进行 MEBB 处理。

结果

与对照组相比，用 120μg/ml MEBB 处理的年轻和老年细胞的生长速度分别增加了 28.1%和 15.2%。MEBB 处理年轻细胞可使 SOD 活性增加 62.5%，而处理老年细胞可使 SOD 活性降低 39.5%。用 MEBB 处理的年轻和老年细胞的过氧化氢酶活性与对照细胞相等。用 MEBB 处理的年轻和老年细胞的 GSH-Px 活性与对照细胞相等。用 120μg/ml MEBB 处理的年轻和老年细胞的 GSH 浓度分别增加了 22.1%和 45.9%。