Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea.
Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Gyeonggi, Korea ; Present address: ST Pharm Co., Siheung 429-912, Gyeonggi, Korea.
AMB Express. 2014 Mar 18;4:15. doi: 10.1186/s13568-014-0015-1. eCollection 2014.
We have reported a transcription profile of an adapted Corynebacterium glutamicum that showed enhanced oxidative stress resistance. To construct an artificial oxidative stress-resistant strain, gene clusters in the β-ketoadipate pathway, which were up-regulated in the adapted strain, were artificially expressed in the wild-type C. glutamicum. The wild-type strain was unable to grow under 2 mM H2O2 containing minimal medium, while the strains expressing pca gene clusters restored growth under the same medium, and the pcaHGBC expression showed the most significant effect among the gene clusters. The expressions of pca gene clusters also enabled the wild-type to increase its resistance against oxidative stressors, such as diamide and cumene hydroperoxide, as well as H2O2. The oxidative stress tolerance of the strain was correlated to the reactive oxygen species (ROS)-scavenging activity of the cell extract. The reason for the enhanced oxidative stress-resistance of C. glutamicum and its applications on the synthetic strain development are discussed.
我们曾报道过一株适应型谷氨酸棒杆菌的转录谱，该菌表现出增强的氧化应激抗性。为构建人工氧化应激抗性菌株，我们在野生型谷氨酸棒杆菌中人工表达了适应型菌株中上调的β-酮戊二酸途径基因簇。野生型菌株在含有 2mM H2O2 的最小培养基中无法生长，而表达 pca 基因簇的菌株在相同培养基中恢复生长，并且 pcaHGBC 表达在基因簇中表现出最显著的效果。pca 基因簇的表达也使野生型菌株能够提高对氧化应激剂（如二酰胺和过氧化枯烯）以及 H2O2 的抗性。该菌株的氧化应激耐受性与细胞提取物的活性氧（ROS）清除活性相关。本文讨论了谷氨酸棒杆菌增强的氧化应激抗性及其在合成菌株开发中的应用。
